Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Characterization of P450 FcpC, the enzyme responsible for bioconversion of diosgenone to isonuatigenone in Streptomyces virginiae IBL-14.

Literature DB >> 19376895

Characterization of P450 FcpC, the enzyme responsible for bioconversion of diosgenone to isonuatigenone in Streptomyces virginiae IBL-14.

Wei Wang¹, Feng-Qing Wang, Dong-Zhi Wei.

Abstract

A new cytochrome P450 monooxygenase, FcpC, from Streptomyces virginiae IBL-14 has been identified. This enzyme is found to be responsible for the bioconversion of a pyrano-spiro steroid (diosgenone) to a rare nuatigenin-type spiro steroid (isonuatigenone), which is a novel C-25-hydroxylated diosgenone derivative. A whole-cell P450 system was developed for the production of isonuatigenone via the expression of the complete three-component electron transfer chain in an Escherichia coli strain.

Entities: Chemical Species

Mesh：

Substances：

Year: 2009 PMID： 19376895 PMCID： PMC2698366 DOI： 10.1128/AEM.02606-08

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

23 in total

1. THE CARBON MONOXIDE-BINDING PIGMENT OF LIVER MICROSOMES. I. EVIDENCE FOR ITS HEMOPROTEIN NATURE.

Authors: T OMURA; R SATO
Journal: J Biol Chem Date: 1964-07 Impact factor: 5.157

2. A new steroidal saponin from Solanum sisymbriifolium roots.

Authors: E A Ferro; N L Alvarenga; D A Ibarrola; M C Hellión-Ibarrola; A G Ravelo
Journal: Fitoterapia Date: 2005-09 Impact factor: 2.882

3. Engineering and analysis of a self-sufficient biosynthetic cytochrome P450 PikC fused to the RhFRED reductase domain.

Authors: Shengying Li; Larissa M Podust; David H Sherman
Journal: J Am Chem Soc Date: 2007-10-04 Impact factor: 15.419

4. Macrolide antibiotic biosynthesis: isolation and properties of two forms of 6-deoxyerythronolide B hydroxylase from Saccharopolyspora erythraea (Streptomyces erythreus).

Authors: A Shafiee; C R Hutchinson
Journal: Biochemistry Date: 1987-09-22 Impact factor: 3.162

5. Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2).

Authors: S D Bentley; K F Chater; A-M Cerdeño-Tárraga; G L Challis; N R Thomson; K D James; D E Harris; M A Quail; H Kieser; D Harper; A Bateman; S Brown; G Chandra; C W Chen; M Collins; A Cronin; A Fraser; A Goble; J Hidalgo; T Hornsby; S Howarth; C-H Huang; T Kieser; L Larke; L Murphy; K Oliver; S O'Neil; E Rabbinowitsch; M-A Rajandream; K Rutherford; S Rutter; K Seeger; D Saunders; S Sharp; R Squares; S Squares; K Taylor; T Warren; A Wietzorrek; J Woodward; B G Barrell; J Parkhill; D A Hopwood
Journal: Nature Date: 2002-05-09 Impact factor: 49.962

6. Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp.

Authors: M Bierman; R Logan; K O'Brien; E T Seno; R N Rao; B E Schoner
Journal: Gene Date: 1992-07-01 Impact factor: 3.688

7. Biocatalytic conversion of avermectin to 4"-oxo-avermectin: heterologous expression of the ema1 cytochrome P450 monooxygenase.

Authors: István Molnár; D Steven Hill; Ross Zirkle; Philip E Hammer; Frank Gross; Thomas G Buckel; Volker Jungmann; Johannes Paul Pachlatko; James M Ligon
Journal: Appl Environ Microbiol Date: 2005-11 Impact factor: 4.792

8. Cytochrome P450105D1 (CYP105D1) from Streptomyces griseus: heterologous expression, activity, and activation effects of multiple xenobiotics.

Authors: M Taylor; D C Lamb; R Cannell; M Dawson; S L Kelly
Journal: Biochem Biophys Res Commun Date: 1999-10-05 Impact factor: 3.575

9. Increasing the efficiency of heterologous promoters in actinomycetes.

Authors: Christopher J Wilkinson; Zoë A Hughes-Thomas; Christine J Martin; Ines Böhm; Tatiana Mironenko; Matthew Deacon; Michael Wheatcroft; Gabriele Wirtz; James Staunton; Peter F Leadlay
Journal: J Mol Microbiol Biotechnol Date: 2002-07

10. Genome analyses of Streptomyces peucetius ATCC 27952 for the identification and comparison of cytochrome P450 complement with other Streptomyces.

Authors: Niranjan Parajuli; Devi B Basnet; Hei Chan Lee; Jae Kyung Sohng; Kwangkyoung Liou
Journal: Arch Biochem Biophys Date: 2004-05-15 Impact factor: 4.013

6 in total

Characterization of P450 FcpC, the enzyme responsible for bioconversion of diosgenone to isonuatigenone in Streptomyces virginiae IBL-14.

1. THE CARBON MONOXIDE-BINDING PIGMENT OF LIVER MICROSOMES. I. EVIDENCE FOR ITS HEMOPROTEIN NATURE.

2. A new steroidal saponin from Solanum sisymbriifolium roots.

3. Engineering and analysis of a self-sufficient biosynthetic cytochrome P450 PikC fused to the RhFRED reductase domain.

4. Macrolide antibiotic biosynthesis: isolation and properties of two forms of 6-deoxyerythronolide B hydroxylase from Saccharopolyspora erythraea (Streptomyces erythreus).

5. Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2).

6. Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp.

7. Biocatalytic conversion of avermectin to 4"-oxo-avermectin: heterologous expression of the ema1 cytochrome P450 monooxygenase.

8. Cytochrome P450105D1 (CYP105D1) from Streptomyces griseus: heterologous expression, activity, and activation effects of multiple xenobiotics.

9. Increasing the efficiency of heterologous promoters in actinomycetes.

10. Genome analyses of Streptomyces peucetius ATCC 27952 for the identification and comparison of cytochrome P450 complement with other Streptomyces.

Review 1. Cytochromes P450 for natural product biosynthesis in Streptomyces: sequence, structure, and function.

Review 2. CYP105-diverse structures, functions and roles in an intriguing family of enzymes in Streptomyces.

Review 3. Reviewing a plethora of oxidative-type reactions catalyzed by whole cells of Streptomyces species.

4. Identification and functional analysis of cytochrome P450 complement in Streptomyces virginiae IBL14.

5. Diosgenone: a second P2(1) polymorph.

Review 6. New Insights on Steroid Biotechnology.