PURPOSE: With breast cancer, early detection and proper staging are critical, and will often influence both the treatment regimen and the therapeutic outcome for those affected with this disease. Improvements in these areas will play a profound role in reducing mortality from breast cancer. EXPERIMENTAL DESIGN: In this work we developed a breast cancer-targeted serotype 5 adenoviral vector, utilizing the tumor-specific mucin-1 promoter in combination with the two-step transcriptional amplification system, a system used to augment the activity of weak tissue-specific promoters. RESULTS: We showed the strong specificity of this tumor-selective adenovirus to express the luciferase optical imaging gene, leading to diagnostic signals that enabled detection of sentinel lymph node metastasis of breast cancer. Furthermore, we were able to target hepatic metastases following systemic administration of this mucin-1 selective virus. CONCLUSIONS: Collectively, we showed that the amplified mucin-1 promoter-driven vector is able to deliver to and selectively express a desirable transgene in metastatic lesions of breast tumors. This work has strong clinical relevance to current diagnostic staging approaches, and could add to targeted therapeutic strategies to advance the fight against breast cancer.
PURPOSE: With breast cancer, early detection and proper staging are critical, and will often influence both the treatment regimen and the therapeutic outcome for those affected with this disease. Improvements in these areas will play a profound role in reducing mortality from breast cancer. EXPERIMENTAL DESIGN: In this work we developed a breast cancer-targeted serotype 5 adenoviral vector, utilizing the tumor-specific mucin-1 promoter in combination with the two-step transcriptional amplification system, a system used to augment the activity of weak tissue-specific promoters. RESULTS: We showed the strong specificity of this tumor-selective adenovirus to express the luciferase optical imaging gene, leading to diagnostic signals that enabled detection of sentinel lymph node metastasis of breast cancer. Furthermore, we were able to target hepatic metastases following systemic administration of this mucin-1 selective virus. CONCLUSIONS: Collectively, we showed that the amplified mucin-1 promoter-driven vector is able to deliver to and selectively express a desirable transgene in metastatic lesions of breast tumors. This work has strong clinical relevance to current diagnostic staging approaches, and could add to targeted therapeutic strategies to advance the fight against breast cancer.
Authors: Martine L M Lamfers; Giulia Fulci; Davide Gianni; Yi Tang; Kazuhiko Kurozumi; Balveen Kaur; Sharif Moeniralm; Yoshinaga Saeki; Jan E Carette; Ralph Weissleder; W Peter Vandertop; Victor W van Beusechem; Clemens M F Dirven; E Antonio Chiocca Journal: Mol Ther Date: 2006-09-22 Impact factor: 11.454
Authors: Xiaoming Xie; Weiya Xia; Zhongkui Li; Hsu-Ping Kuo; Yuanfang Liu; Zheng Li; Qingqing Ding; Su Zhang; Bill Spohn; Yan Yang; Yongkun Wei; Jing-Yu Lang; Douglas B Evans; Paul J Chiao; James L Abbruzzese; Mien-Chie Hung Journal: Cancer Cell Date: 2007-07 Impact factor: 31.743
Authors: Ebba Brakenhielm; Jeremy B Burton; Mai Johnson; Nelson Chavarria; Kouki Morizono; Irvin Chen; Kari Alitalo; Lily Wu Journal: Int J Cancer Date: 2007-11-15 Impact factor: 7.396
Authors: Hua-Jung Li; Maaike Everts; Larisa Pereboeva; Svetlana Komarova; Anat Idan; David T Curiel; Harvey R Herschman Journal: Cancer Res Date: 2007-06-01 Impact factor: 12.701
Authors: Isabelle Soerjomataram; Marieke W J Louwman; Jacques G Ribot; Jan A Roukema; Jan Willem W Coebergh Journal: Breast Cancer Res Treat Date: 2007-03-22 Impact factor: 4.872
Authors: John A Ronald; Hui-Yen Chuang; Anca Dragulescu-Andrasi; Sharon S Hori; Sanjiv S Gambhir Journal: Proc Natl Acad Sci U S A Date: 2015-02-23 Impact factor: 11.205
Authors: Kwang Il Kim; Yong Jin Lee; Tae Sup Lee; Inho Song; Gi Jeong Cheon; Sang Moo Lim; June-Key Chung; Joo Hyun Kang Journal: Nucl Med Mol Imaging Date: 2012-09-06