PURPOSE: Human microRNA-141 (miR-141), a member of the miR-200 family, has been reported to be associated with various human malignancies. However, it remains unknown whether miR-141 is involved in the pathogenesis of gastric cancer. Therefore, we examined the expression of miR-141 in gastric cancer tissues and the effect of miR-141 overexpression on cancer cell proliferation. METHODS: The expression level of miR-141 in 35 pair-matched gastric neoplastic and adjacent non-neoplastic tissues, and in 5 gastric cancer cell lines were examined by quantitative real-time PCR. The growth of MGC-803 cells transfected with miRNA precursor was examined by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazoliumbromide) assay. RESULTS: MiR-141 was significantly down-regulated in 80% (28/35) of primary gastric cancer tissues compared with pair-matched adjacent non-tumor tissues (P < 0.01). The expression of miR-141 was also found to be substantially reduced in several human gastric cancer cell lines such as MGC-803, HGC-27, SGC-7901 and BGC-823 cells. Overexpression of miR-141 with its precursors significantly inhibited the proliferation of gastric cancer cells. CONCLUSIONS: These results suggest that miR-141 may be involved in the development of gastric cancer through its inhibitory effect on cell proliferation.
PURPOSE:HumanmicroRNA-141 (miR-141), a member of the miR-200 family, has been reported to be associated with various humanmalignancies. However, it remains unknown whether miR-141 is involved in the pathogenesis of gastric cancer. Therefore, we examined the expression of miR-141 in gastric cancer tissues and the effect of miR-141 overexpression on cancer cell proliferation. METHODS: The expression level of miR-141 in 35 pair-matched gastric neoplastic and adjacent non-neoplastic tissues, and in 5 gastric cancer cell lines were examined by quantitative real-time PCR. The growth of MGC-803 cells transfected with miRNA precursor was examined by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazoliumbromide) assay. RESULTS:MiR-141 was significantly down-regulated in 80% (28/35) of primary gastric cancer tissues compared with pair-matched adjacent non-tumor tissues (P < 0.01). The expression of miR-141 was also found to be substantially reduced in several humangastric cancer cell lines such as MGC-803, HGC-27, SGC-7901 and BGC-823 cells. Overexpression of miR-141 with its precursors significantly inhibited the proliferation of gastric cancer cells. CONCLUSIONS: These results suggest that miR-141 may be involved in the development of gastric cancer through its inhibitory effect on cell proliferation.
Authors: Marilena V Iorio; Rosa Visone; Gianpiero Di Leva; Valentina Donati; Fabio Petrocca; Patrizia Casalini; Cristian Taccioli; Stefano Volinia; Chang-Gong Liu; Hansjuerg Alder; George A Calin; Sylvie Ménard; Carlo M Croce Journal: Cancer Res Date: 2007-09-15 Impact factor: 12.701
Authors: George Adrian Calin; Cinzia Sevignani; Calin Dan Dumitru; Terry Hyslop; Evan Noch; Sai Yendamuri; Masayoshi Shimizu; Sashi Rattan; Florencia Bullrich; Massimo Negrini; Carlo M Croce Journal: Proc Natl Acad Sci U S A Date: 2004-02-18 Impact factor: 11.205
Authors: Yong Guo; Zhaoli Chen; Liang Zhang; Fang Zhou; Susheng Shi; Xiaoli Feng; Baozhong Li; Xin Meng; Xi Ma; Mingyong Luo; Kang Shao; Ning Li; Bin Qiu; Keith Mitchelson; Jing Cheng; Jie He Journal: Cancer Res Date: 2008-01-01 Impact factor: 12.701
Authors: Laura Gramantieri; Manuela Ferracin; Francesca Fornari; Angelo Veronese; Silvia Sabbioni; Chang-Gong Liu; George A Calin; Catia Giovannini; Eros Ferrazzi; Gian Luca Grazi; Carlo M Croce; Luigi Bolondi; Massimo Negrini Journal: Cancer Res Date: 2007-07-01 Impact factor: 12.701
Authors: Cameron M Smith; David I Watson; Mary P Leong; George C Mayne; Michael Z Michael; Bas P L Wijnhoven; Damian J Hussey Journal: World J Gastroenterol Date: 2011-02-28 Impact factor: 5.742
Authors: Wellerson R Scarano; Amina Bedrat; Luiz G Alonso-Costa; Ariana M Aquino; Bruno Fantinatti; Luis A Justulin; Luis F Barbisan; Paula P Freire; Jodi A Flaws; Lemos Bernardo Journal: Toxicol Sci Date: 2019-06-14 Impact factor: 4.849
Authors: Amanda L Treece; Daniel L Duncan; Weihua Tang; Sandra Elmore; Douglas R Morgan; Ricardo L Dominguez; Olga Speck; Michael O Meyers; Margaret L Gulley Journal: Lab Invest Date: 2016-03-07 Impact factor: 5.662
Authors: Lukas Vrba; Taylor J Jensen; James C Garbe; Ronald L Heimark; Anne E Cress; Sally Dickinson; Martha R Stampfer; Bernard W Futscher Journal: PLoS One Date: 2010-01-13 Impact factor: 3.240
Authors: Michael F Gallagher; Richard J Flavin; Salah A Elbaruni; Jamie K McInerney; Paul C Smyth; Yvonne M Salley; Sebastian F Vencken; Sharon A O'Toole; Alexandros Laios; Mathia Yc Lee; Karen Denning; Jinghuan Li; Sinead T Aherne; Kai Q Lao; Cara M Martin; Orla M Sheils; John J O'Leary Journal: J Ovarian Res Date: 2009-12-16 Impact factor: 4.234