Literature DB >> 19357211

Validation of five-colony pool analysis using multiplex real-time PCR for detection of diarrheagenic Escherichia coli.

F Barletta1, T J Ochoa, L Ecker, A I Gil, C F Lanata, T G Cleary.   

Abstract

Five Escherichia coli colonies/patient were studied to evaluate the reliability of a multiplex real-time PCR assay for detection of diarrheagenic Escherichia coli groups, using a pool of five colonies rather than individual colonies. Sensitivity and specificity were 98% and 100%, respectively, at a fifth of the cost of the individual colony analysis.

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Year:  2009        PMID： 19357211      PMCID： PMC2691103          DOI： 10.1128/JCM.00608-09

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


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