| Literature DB >> 19348733 |
Martin J Reid1, Alison K Cross, Gail Haddock, Stuart M Allan, Chris J Stock, M Nicola Woodroofe, David J Buttle, Rowena A D Bunning.
Abstract
The ADAMTS enzymes (a disintegrin and metalloproteinase with thrombospondin type 1-like motifs) have important roles in central nervous system (CNS) physiology and pathology. This current study aimed to analyse the expression of ADAMTS-9 following transient middle cerebral artery occlusion (tMCAo) in the rat, a model of focal cerebral ischaemia. Using real-time RT-PCR, ADAMTS-9 mRNA was demonstrated to be significantly up-regulated in tMCAo brain tissue compared to sham-operated at 24h post-ischaemia. The mature form of the ADAMTS-9 protein was only detected by Western blotting in brains subjected to tMCAo at 24h. In situ hybridisation demonstrated that ADAMTS-9 mRNA was expressed by neurones in tMCAo tissue. This study indicates that ADAMTS-9 expression is modulated in response to cerebral ischaemia in vivo and further research will resolve whether it plays a role in the subsequent degenerative or repair processes.Entities:
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Year: 2009 PMID： 19348733 DOI： 10.1016/j.neulet.2009.01.058
Source DB: PubMed Journal: Neurosci Lett ISSN： 0304-3940 Impact factor: 3.046