Coenzyme Q is an isoprenylated benzoquinone lipid that functions in respiratory electron transport and as a lipid antioxidant. Dietary supplementation with Q is increasingly used as a therapeutic for treatment of mitochondrial and neurodegenerative diseases, yet little is known regarding the mechanism of its uptake. As opposed to other yeast backgrounds, EG103 strains are unable to import exogenous Q(6) to the mitochondria. Furthermore, the distribution of exogenous Q(6) among endomembranes suggests an impairment of the membrane traffic at the level of the endocytic pathway. This fact was confirmed after the detection of defects in the incorporation of FM4-64 marker and CPY delivery to the vacuole. A similar effect was demonstrated in double mutant strains in Q(6) synthesis and several steps of endocytic process; those cells are unable to uptake exogenous Q(6) to the mitochondria and restore the growth on non-fermentable carbon sources. Additional data about the positive effect of peptone presence for exogenous Q(6) uptake support the hypothesis that Q(6) is transported to mitochondria through an endocytic-based system.
Coenzyme Q is an isoprenylated n class="Chemical">benzoquinone lipid that functions in respiratory electron transport and as a lipid antioxidant. Dietary supplementation with Q is increasingly used as a therapeutic for treatment of mitochondrial and neurodegenerative diseases, yet little is known regarding the mechanism of its uptake. As opposed to other yeast backgrounds, EG103 strains are unable to import exogenous Q(6) to the mitochondria. Furthermore, the distribution of exogenous Q(6) among endomembranes suggests an impairment of the membrane traffic at the level of the endocytic pathway. This fact was confirmed after the detection of defects in the incorporation of FM4-64 marker and CPY delivery to the vacuole. A similar effect was demonstrated in double mutant strains in Q(6) synthesis and several steps of endocytic process; those cells are unable to uptake exogenous Q(6) to the mitochondria and restore the growth on non-fermentable carbon sources. Additional data about the positive effect of peptone presence for exogenous Q(6) uptake support the hypothesis that Q(6) is transported to mitochondria through an endocytic-based system.
Authors: Cuiwen H He; Dylan S Black; Theresa P T Nguyen; Charles Wang; Chandra Srinivasan; Catherine F Clarke Journal: Biochim Biophys Acta Date: 2015-05-23
Authors: Courtney M Anderson; Melissa Kazantzis; Jinshan Wang; Subramaniam Venkatraman; Renata L S Goncalves; Casey L Quinlan; Ryan Ng; Martin Jastroch; Daniel I Benjamin; Biao Nie; Candice Herber; An-Angela Ngoc Van; Michael J Park; Dawee Yun; Karen Chan; Angela Yu; Peter Vuong; Maria Febbraio; Daniel K Nomura; Joseph L Napoli; Martin D Brand; Andreas Stahl Journal: Cell Rep Date: 2015-01-22 Impact factor: 9.423
Authors: Saskia F Heeringa; Gil Chernin; Moumita Chaki; Weibin Zhou; Alexis J Sloan; Ziming Ji; Letian X Xie; Leonardo Salviati; Toby W Hurd; Virginia Vega-Warner; Paul D Killen; Yehoash Raphael; Shazia Ashraf; Bugsu Ovunc; Dominik S Schoeb; Heather M McLaughlin; Rannar Airik; Christopher N Vlangos; Rasheed Gbadegesin; Bernward Hinkes; Pawaree Saisawat; Eva Trevisson; Mara Doimo; Alberto Casarin; Vanessa Pertegato; Gianpietro Giorgi; Holger Prokisch; Agnès Rötig; Gudrun Nürnberg; Christian Becker; Su Wang; Fatih Ozaltin; Rezan Topaloglu; Aysin Bakkaloglu; Sevcan A Bakkaloglu; Dominik Müller; Antje Beissert; Sevgi Mir; Afig Berdeli; Seza Varpizen; Martin Zenker; Verena Matejas; Carlos Santos-Ocaña; Placido Navas; Takehiro Kusakabe; Andreas Kispert; Sema Akman; Neveen A Soliman; Stefanie Krick; Peter Mundel; Jochen Reiser; Peter Nürnberg; Catherine F Clarke; Roger C Wiggins; Christian Faul; Friedhelm Hildebrandt Journal: J Clin Invest Date: 2011-04-11 Impact factor: 14.808
Authors: Cuiwen H He; Dylan S Black; Christopher M Allan; Brigitte Meunier; Shamima Rahman; Catherine F Clarke Journal: Front Physiol Date: 2017-07-07 Impact factor: 4.566
Authors: Lucía Fernández-Del-Río; Miranda E Kelly; Jaime Contreras; Michelle C Bradley; Andrew M James; Michael P Murphy; Gregory S Payne; Catherine F Clarke Journal: Free Radic Biol Med Date: 2020-05-06 Impact factor: 7.376
Authors: Courtney W Stairs; Laura Eme; Sergio A Muñoz-Gómez; Alejandro Cohen; Graham Dellaire; Jennifer N Shepherd; James P Fawcett; Andrew J Roger Journal: Elife Date: 2018-04-26 Impact factor: 8.140
Authors: Ignasi B Verdaguer; Camila A Zafra; Marcell Crispim; Rodrigo A C Sussmann; Emília A Kimura; Alejandro M Katzin Journal: Molecules Date: 2019-10-16 Impact factor: 4.411