| Literature DB >> 19345326 |
Kexin Xu1, Hermela Shimelis, Douglas E Linn, Richeng Jiang, Xi Yang, Feng Sun, Zhiyong Guo, Hege Chen, Wei Li, Hegang Chen, Xiangtian Kong, Jonathan Melamed, Shengyun Fang, Zhen Xiao, Timothy D Veenstra, Yun Qiu.
Abstract
The androgen receptor (AR) plays a critical role in prostate cancer. We have identified a ubiquitin E3 ligase, RNF6, as an AR-associated protein in a proteomic screen. RNF6 induces AR ubiquitination and promotes AR transcriptional activity. Specific knockdown of RNF6 or mutation of RNF6-induced ubiquitination acceptor sites on AR selectively alters expression of a subset of AR target genes and diminishes recruitment of AR and its coactivators to androgen-responsive elements present in the regulatory region of these genes. Furthermore, RNF6 is overexpressed in hormone-refractory human prostate cancer tissues and required for prostate cancer cell growth under androgen-depleted conditions. Our data suggest that RNF6-induced ubiquitination may regulate AR transcriptional activity and specificity through modulating cofactor recruitment.Entities:
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Year: 2009 PMID： 19345326 PMCID： PMC2848969 DOI： 10.1016/j.ccr.2009.02.021
Source DB: PubMed Journal: Cancer Cell ISSN： 1535-6108 Impact factor: 31.743