Literature DB >> 19343536

Solid phase subtractive cloning in differentially expressed genes identification.

Oscar F D'Urso1, Pietro I D'Urso, Carlo Storelli, Santo Marsigliante.   

Abstract

We developed an array-based subtractive hybridization system for one-step high-throughput subtraction. We printed subtractor RNA up to 10.000 times obtaining an excellent contact surface using a little amount of RNA. During hybridization cDNA, common to subtractor and target samples, remains attached to slide immobilized RNA, leaving free in solution target specific cDNA which after retrieval is cloned.

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Year:  2009        PMID： 19343536     DOI： 10.1007/s11033-009-9530-6

Source DB:  PubMed          Journal:  Mol Biol Rep        ISSN： 0301-4851            Impact factor:   2.316


  16 in total

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Journal:  Annu Rev Biophys Bioeng       Date:  1976

2.  A novel method for the isolation of tissue-specific genes.

Authors:  I R Rodriguez; G J Chader
Journal:  Nucleic Acids Res       Date:  1992-07-11       Impact factor: 16.971

3.  Analysis of differentially expressed genes in response to bacterial stimulation in hemocytes of the carpet-shell clam Ruditapes decussatus: identification of new antimicrobial peptides.

Authors:  Camino Gestal; Marímar Costa; Antonio Figueras; Beatriz Novoa
Journal:  Gene       Date:  2007-08-15       Impact factor: 3.688

4.  Molecular cloning and characterization of phosphate (Pi) responsive genes in Gulf ryegrass (Lolium multiflorum L.): a Pi hyperaccumulator.

Authors:  Perumal Venkatachalam; Ajay Jain; Shivendra Sahi; Kashchandra Raghothama
Journal:  Plant Mol Biol       Date:  2008-09-28       Impact factor: 4.076

5.  An analysis of differential display shows a strong bias towards high copy number mRNAs.

Authors:  D J Bertioli; U H Schlichter; M J Adams; P R Burrows; H H Steinbiss; J F Antoniw
Journal:  Nucleic Acids Res       Date:  1995-11-11       Impact factor: 16.971

Review 6.  Subtractive cloning: past, present, and future.

Authors:  C G Sagerström; B I Sun; H L Sive
Journal:  Annu Rev Biochem       Date:  1997       Impact factor: 23.643

7.  A subtractive hybridization method to isolate tissue-specific transcripts: application to the selection of new brain-specific products.

Authors:  D Maréchal; C Forceille; D Breyer; D Delapierre; A Dresse
Journal:  Anal Biochem       Date:  1993-02-01       Impact factor: 3.365

8.  WAF1, a potential mediator of p53 tumor suppression.

Authors:  W S el-Deiry; T Tokino; V E Velculescu; D B Levy; R Parsons; J M Trent; D Lin; W E Mercer; K W Kinzler; B Vogelstein
Journal:  Cell       Date:  1993-11-19       Impact factor: 41.582

9.  Activation of mouse genes in transformed cells.

Authors:  M R Scott; K H Westphal; P W Rigby
Journal:  Cell       Date:  1983-09       Impact factor: 41.582

10.  Subtractive cDNA cloning using oligo(dT)30-latex and PCR: isolation of cDNA clones specific to undifferentiated human embryonal carcinoma cells.

Authors:  E Hara; T Kato; S Nakada; S Sekiya; K Oda
Journal:  Nucleic Acids Res       Date:  1991-12       Impact factor: 16.971
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  1 in total

1.  Applying Subtractive Hybridization Technique to Enrich and Amplify Tumor-Specific Transcripts of Esophageal Squamous Cell Carcinoma.

Authors:  Reihaneh Alsadat Mahmoudian; Mohammad Reza Abbaszadegan; Mehran Gholamin
Journal:  Pathol Oncol Res       Date:  2016-07-15       Impact factor: 3.201
  1 in total

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