Literature DB >> 19342782

Overexpression, purification and crystallization of a thermostable DNA ligase from the archaeon Thermococcus sp. 1519.

E Y Bezsudnova1, M V Kovalchuk, A V Mardanov, K M Poliakov, V O Popov, N V Ravin, K G Skryabin, V A Smagin, T N Stekhanova, T V Tikhonova.   

Abstract

DNA ligases catalyze the sealing of 5'-phosphate and 3'-hydroxyl termini at single-strand breaks in double-stranded DNA and their function is essential to maintain the integrity of the genome in DNA metabolism. An ATP-dependent DNA ligase from the archaeon Thermococcus sp. 1519 was overexpressed, purified and crystallized. Crystals were obtained using the hanging-drop vapour-diffusion method employing 35%(v/v) Tacsimate pH 7.0 as a precipitant and diffracted X-rays to 3.09 A resolution. They belonged to space group P4(1)2(1)2, with unit-cell parameters a = b = 79.7, c = 182.6 A.

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Year:  2009        PMID： 19342782      PMCID： PMC2664762          DOI： 10.1107/S1744309109007799

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  13 in total

1.  A DNA ligase from a hyperthermophilic archaeon with unique cofactor specificity.

Authors:  M Nakatani; S Ezaki; H Atomi; T Imanaka
Journal:  J Bacteriol       Date:  2000-11       Impact factor: 3.490

2.  REFMAC5 dictionary: organization of prior chemical knowledge and guidelines for its use.

Authors:  Alexei A Vagin; Roberto A Steiner; Andrey A Lebedev; Liz Potterton; Stuart McNicholas; Fei Long; Garib N Murshudov
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2004-11-26

3.  The closed structure of an archaeal DNA ligase from Pyrococcus furiosus.

Authors:  Hirokazu Nishida; Shinichi Kiyonari; Yoshizumi Ishino; Kosuke Morikawa
Journal:  J Mol Biol       Date:  2006-06-12       Impact factor: 5.469

4.  A flexible interface between DNA ligase and PCNA supports conformational switching and efficient ligation of DNA.

Authors:  John M Pascal; Oleg V Tsodikov; Greg L Hura; Wei Song; Elizabeth A Cotner; Scott Classen; Alan E Tomkinson; John A Tainer; Tom Ellenberger
Journal:  Mol Cell       Date:  2006-10-20       Impact factor: 17.970

Review 5.  DNA and RNA ligases: structural variations and shared mechanisms.

Authors:  John M Pascal
Journal:  Curr Opin Struct Biol       Date:  2008-02-08       Impact factor: 6.809

6.  [Isolation and characteristics of new thermostable DNA ligase from archaea of the genus Thermococcus].

Authors:  V A Smagin; A V Mardanov; E A Bonch-Osmolovskaia; N V Ravin
Journal:  Prikl Biokhim Mikrobiol       Date:  2008 Sep-Oct

7.  Biochemical characterization of an ATP-dependent DNA ligase from the hyperthermophilic crenarchaeon Sulfolobus shibatae.

Authors:  Xiaoqin Lai; Hongbing Shao; Fuying Hao; Li Huang
Journal:  Extremophiles       Date:  2002-07-30       Impact factor: 2.395

8.  Characterization of an ATP-dependent DNA ligase from the thermophilic archaeon Methanobacterium thermoautotrophicum.

Authors:  V Sriskanda; Z Kelman; J Hurwitz; S Shuman
Journal:  Nucleic Acids Res       Date:  2000-06-01       Impact factor: 16.971

Review 9.  DNA ligase: structure, mechanism, and function.

Authors:  I R Lehman
Journal:  Science       Date:  1974-11-29       Impact factor: 47.728

10.  Characterization of a thermophilic DNA ligase from the archaeon Thermococcus fumicolans.

Authors:  Jean-luc Rolland; Yannick Gueguen; Cècile Persillon; Jean-Michel Masson; Jacques Dietrich
Journal:  FEMS Microbiol Lett       Date:  2004-07-15       Impact factor: 2.742
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  3 in total

1.  Expression, purification, crystallization and preliminary crystallographic analysis of a thermostable DNA ligase from the archaeon Thermococcus sibiricus.

Authors:  T E Petrova; E Y Bezsudnova; B D Dorokhov; E S Slutskaya; K M Polyakov; P V Dorovatovskiy; N V Ravin; K G Skryabin; M V Kovalchuk; V O Popov
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2012-01-25

2.  ATP-dependent DNA ligase from Thermococcus sp. 1519 displays a new arrangement of the OB-fold domain.

Authors:  T Petrova; E Y Bezsudnova; K M Boyko; A V Mardanov; K M Polyakov; V V Volkov; M Kozin; N V Ravin; I G Shabalin; K G Skryabin; T N Stekhanova; M V Kovalchuk; V O Popov
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2012-11-14

Review 3.  Archaeal Nucleic Acid Ligases and Their Potential in Biotechnology.

Authors:  Cecilia R Chambers; Wayne M Patrick
Journal:  Archaea       Date:  2015-10-01       Impact factor: 3.273
  3 in total

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