Molecular cloning, sequence characterization and tissue transcription profile analyses of two novel genes: LCK and CDK2 from the Black-boned sheep (Ovis aries).

The complete coding sequences of two sheep genes--LCK and CDK2--were amplified using the rapid amplification of cDNA ends method based on three sheep EST sequences whose translated amino acids contain the domain PTKc_Lck_BIk and S_TKc domain, respectively. The sequence analyses of these two genes revealed that the sheep LCK gene encodes a protein of 509 amino acids which has high homology with the lymphocyte-specific protein tyrosine kinase (LCK) of eight species: bovine (99%), human (96%), dog (96%), Aotus nancymaae (95%), mouse (94%), rat (91%), horse (91%) and chicken (81%). The sheep CDK2 gene encodes a protein of 298 amino acids which has high homology with the cyclin-dependent kinase 2 (CDK2) of ten species: bovine (100%), goat (100%), rat (99%), mouse (99%), Chinese hamster (99%), dog (98%), golden hamster (98%), human (98%), horse (98%) and rhesus monkey (98%). The tissue transcription profile analyses indicated that that the Black-boned sheep LCK and CDK2 genes are generally but differentially expressed in the detected tissues including in tissues including spleen, muscle, skin, kidney, lung, liver and heart. These data serve as a foundation for further insight into these two genes.