OBJECTIVE AND DESIGN: It is believed that correction of membrane fatty acid deficiency in cystic fibrosis (CF) downregulates the synthesis of proinflammatory mediators. We tested the hypothesis that an increase of the proportion of docosahexaenoic acid (DHA) in the membrane in vitro changes the neutrophil response to Pseudomonas aeruginosa lipopolysaccharide (LPS). RESULTS: Treatment with DHA increased the secretion of interleukin(IL)-1|*alpha*| by CF neutrophils, but the secretion of other cytokines, CD11b expression, and arachidonic acid (AA) release were not affected either in CF or control (CT) neutrophils. Both with and without DHA, only one out of eight CF neutrophils responded to LPS with an increase of released AA, while five out of seven CT cells released more AA (CF vs. CT P < 0.05 by Fisher test). CONCLUSIONS: These results indicate that in neutrophils the beneficial effects of DHA on immune response are not directly related to the generation of proinflammatory precursors, and suggest that in CF the lower neutrophil AA generation in response to activation could cause insufficient production of lipid mediators involved in the resolution of lung inflammation.
OBJECTIVE AND DESIGN: It is believed that correction of membrane fatty acid deficiency in cystic fibrosis (CF) downregulates the synthesis of proinflammatory mediators. We tested the hypothesis that an increase of the proportion of docosahexaenoic acid (DHA) in the membrane in vitro changes the neutrophil response to Pseudomonas aeruginosalipopolysaccharide (LPS). RESULTS: Treatment with DHA increased the secretion of interleukin(IL)-1|*alpha*| by CF neutrophils, but the secretion of other cytokines, CD11b expression, and arachidonic acid (AA) release were not affected either in CF or control (CT) neutrophils. Both with and without DHA, only one out of eight CF neutrophils responded to LPS with an increase of released AA, while five out of seven CT cells released more AA (CF vs. CT P < 0.05 by Fisher test). CONCLUSIONS: These results indicate that in neutrophils the beneficial effects of DHA on immune response are not directly related to the generation of proinflammatory precursors, and suggest that in CF the lower neutrophil AA generation in response to activation could cause insufficient production of lipid mediators involved in the resolution of lung inflammation.
Authors: Isabel Calvo Del Castillo; Juan G Alvarez; Steven D Freedman; Mario Ollero; Leonardo Claros; J Cecilia Song; James Yoo; Jeffrey B Matthews Journal: J Surg Res Date: 2003-04 Impact factor: 2.192
Authors: Steven D Freedman; Paola G Blanco; Munir M Zaman; Julie C Shea; Mario Ollero; Isabel K Hopper; Deborah A Weed; Andres Gelrud; Meredith M Regan; Michael Laposata; Juan G Alvarez; Brian P O'Sullivan Journal: N Engl J Med Date: 2004-02-05 Impact factor: 91.245
Authors: Joo Y Lee; Anthony Plakidas; Won H Lee; Anne Heikkinen; Prithiva Chanmugam; George Bray; Daniel H Hwang Journal: J Lipid Res Date: 2002-12-01 Impact factor: 5.922
Authors: Jesmond Dalli; Guglielmo Rosignoli; Richard P G Hayhoe; Aleksander Edelman; Mauro Perretti Journal: Am J Pathol Date: 2010-05-20 Impact factor: 4.307