Literature DB >> 19328803

A streamlined method for rapid and sensitive chromatin immunoprecipitation.

Michael L Sikes1, Justin M Bradshaw, Wendell T Ivory, Jessica L Lunsford, Ruth E McMillan, Clayton R Morrison.   

Abstract

We report a streamlined procedure to efficiently carry samples from chromatin to qPCR-compatible DNA in as little as 4 h. We use this streamlined ChIP to quantify histone H3 modifications at active (cad) and repressed (T early alpha) promoters in a Rag1-deficient pro-T cell line after 1-2 h IP. We further show that the protocol readily quantified histone modifications in chromatin from 10(4) Rag-deficient DN thymocytes. Taken together, these data outline a simple, cost-effective procedure for efficient ChIP analysis.

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Year:  2009        PMID： 19328803      PMCID： PMC2692951          DOI： 10.1016/j.jim.2009.03.007

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  20 in total

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  9 in total

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