| Literature DB >> 19328766 |
Ingo H Engels1, Catherine Daguia, Truc Huynh, Hugo Urbina, Jörg Buddenkotte, Andrew Schumacher, Jeremy S Caldwell, Achim Brinker.
Abstract
Neural precursor cell expressed, developmentally down-regulated gene 8 (NEDD8) is a recently discovered ubiquitin-like posttranslational modifier. NEDD8 acts predominantly as a regulator of ubiquitin-protein ligases and as a decoy for proteins targeted for proteasomal degradation. It thereby controls key events in cell cycle progression and embryogenesis. Deneddylase-1 (DEN1/NEDP1/SENP8) features a selective peptidase activity converting the proNEDD8 precursor to its mature form and an isopeptidase activity deconjugating NEDD8 from substrates such as cullins and p53. In this study, we describe a high-throughput screening (HTS)-compatible time-resolved fluorescent resonance energy transfer (TR-FRET) assay measuring the peptidase activity of DEN1.Entities:
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Year: 2009 PMID： 19328766 DOI： 10.1016/j.ab.2009.03.035
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365