OBJECTIVE: The purpose of this study was to investigate the effects of environmental tobacco smoke (ETS) and house dust mite allergen (HDMA) on the laryngeal mucosa of guinea pigs. STUDY DESIGN: Prospective, controlled animal study. SUBJECTS AND METHODS: Sixteen juvenile guinea pigs were randomly assigned to one of four treatment groups: (1) filtered air, (2) HDMA, (3) ETS, and (4) ETS and HDMA. The mucosal response of retrieved larynges was measured by eosinophil grade and stored mucin index. RESULTS: HDMA exposure increased eosinophil grade in the supraglottis. The mean eosinophil grade in the supraglottis was 1.25 (+/-1.26) in the filtered air group. The grade increased to 3.60 (+/-0.89) in the HDMA group (P = 0.021) and 3.00 (+/-1.16) in the ETS/HDMA group (P = 0.078). HDMA and ETS exposure was associated with increased mucin index in the subglottis. The grade increased from 1.50 (+/-1.73) in the control group to 3.25 (+/-0.50) in the ETS, 2.80 (+/-1.64) in the HDMA, and 3.25 (+/-0.50) in the ETS/HDMA group (P < 0.05). CONCLUSION: Exposure to HDMA was associated with eosinophilia in the supraglottis. Exposure to HDMA and ETS was associated with elevated mucin in the subglottis. These results provide further evidence that ETS and inhaled allergens may play a role in the development of chronic laryngitis.
OBJECTIVE: The purpose of this study was to investigate the effects of environmental tobacco smoke (ETS) and house dust mite allergen (HDMA) on the laryngeal mucosa of guinea pigs. STUDY DESIGN: Prospective, controlled animal study. SUBJECTS AND METHODS: Sixteen juvenile guinea pigs were randomly assigned to one of four treatment groups: (1) filtered air, (2) HDMA, (3) ETS, and (4) ETS and HDMA. The mucosal response of retrieved larynges was measured by eosinophil grade and stored mucin index. RESULTS: HDMA exposure increased eosinophil grade in the supraglottis. The mean eosinophil grade in the supraglottis was 1.25 (+/-1.26) in the filtered air group. The grade increased to 3.60 (+/-0.89) in the HDMA group (P = 0.021) and 3.00 (+/-1.16) in the ETS/HDMA group (P = 0.078). HDMA and ETS exposure was associated with increased mucin index in the subglottis. The grade increased from 1.50 (+/-1.73) in the control group to 3.25 (+/-0.50) in the ETS, 2.80 (+/-1.64) in the HDMA, and 3.25 (+/-0.50) in the ETS/HDMA group (P < 0.05). CONCLUSION: Exposure to HDMA was associated with eosinophilia in the supraglottis. Exposure to HDMA and ETS was associated with elevated mucin in the subglottis. These results provide further evidence that ETS and inhaled allergens may play a role in the development of chronic laryngitis.
Authors: Sarah K Wise; Sandra Y Lin; Elina Toskala; Richard R Orlandi; Cezmi A Akdis; Jeremiah A Alt; Antoine Azar; Fuad M Baroody; Claus Bachert; G Walter Canonica; Thomas Chacko; Cemal Cingi; Giorgio Ciprandi; Jacquelynne Corey; Linda S Cox; Peter Socrates Creticos; Adnan Custovic; Cecelia Damask; Adam DeConde; John M DelGaudio; Charles S Ebert; Jean Anderson Eloy; Carrie E Flanagan; Wytske J Fokkens; Christine Franzese; Jan Gosepath; Ashleigh Halderman; Robert G Hamilton; Hans Jürgen Hoffman; Jens M Hohlfeld; Steven M Houser; Peter H Hwang; Cristoforo Incorvaia; Deborah Jarvis; Ayesha N Khalid; Maritta Kilpeläinen; Todd T Kingdom; Helene Krouse; Desiree Larenas-Linnemann; Adrienne M Laury; Stella E Lee; Joshua M Levy; Amber U Luong; Bradley F Marple; Edward D McCoul; K Christopher McMains; Erik Melén; James W Mims; Gianna Moscato; Joaquim Mullol; Harold S Nelson; Monica Patadia; Ruby Pawankar; Oliver Pfaar; Michael P Platt; William Reisacher; Carmen Rondón; Luke Rudmik; Matthew Ryan; Joaquin Sastre; Rodney J Schlosser; Russell A Settipane; Hemant P Sharma; Aziz Sheikh; Timothy L Smith; Pongsakorn Tantilipikorn; Jody R Tversky; Maria C Veling; De Yun Wang; Marit Westman; Magnus Wickman; Mark Zacharek Journal: Int Forum Allergy Rhinol Date: 2018-02 Impact factor: 3.858
Authors: Rena Yadlapati; John E Pandolfino; Alcina K Lidder; Nadine Shabeeb; Diana-Marie Jaiyeola; Christopher Adkins; Neelima Agrawal; Andrew Cooper; Caroline P E Price; Jody D Ciolino; Andrew J Gawron; Stephanie S Smith; Michiel Bove; Bruce K Tan Journal: Am J Gastroenterol Date: 2016-04-19 Impact factor: 10.864