Literature DB >> 19323530

Facilitated assignment of adenine H2 resonances in oligonucleotides using homonuclear long-range couplings.

Jin Zhang1, Alexander Spring, Markus W Germann.   

Abstract

The small homonuclear coupling constant between H2 and H8 protons of adenine (0.5 +/- 0.2 Hz) was determined and exploited to assign the H2 proton of adenine residues in DNA oligonucleotides. The utility of this approach is demonstrated for two different DNA sequences in D(2)O, for which we show a rapid and unambiguous assignment of the A H2 resonances.

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Year:  2009        PMID: 19323530      PMCID: PMC2889177          DOI: 10.1021/ja900771b

Source DB:  PubMed          Journal:  J Am Chem Soc        ISSN: 0002-7863            Impact factor:   15.419


  5 in total

Review 1.  RNA structure and NMR spectroscopy.

Authors:  G Varani; I Tinoco
Journal:  Q Rev Biophys       Date:  1991-11       Impact factor: 5.318

2.  Asymmetry in 13C-13C COSY spectra provides information on ligand geometry in paramagnetic proteins.

Authors:  Ivano Bertini; Beatriz Jiménez; Mario Piccioli; Luisa Poggi
Journal:  J Am Chem Soc       Date:  2005-09-07       Impact factor: 15.419

3.  A TROSY relayed HCCH-COSY experiment for correlating adenine H2/H8 resonances in uniformly 13C-labeled RNA molecules.

Authors:  B Simon; K Zanier; M Sattler
Journal:  J Biomol NMR       Date:  2001-06       Impact factor: 2.835

Review 4.  Water and monovalent ions in the minor groove of B-DNA oligonucleotides as seen by NMR.

Authors:  B Halle; V P Denisov
Journal:  Biopolymers       Date:  1998       Impact factor: 2.505

5.  Through-bond correlation of adenine H2 and H8 protons in unlabeled DNA fragments by HMBC spectroscopy.

Authors:  M J van Dongen; S S Wijmenga; R Eritja; F Azorín; C W Hilbers
Journal:  J Biomol NMR       Date:  1996-09       Impact factor: 2.835

  5 in total

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