Literature DB >> 19321359

Identifying nonspecific ligand binding in electrospray ionization mass spectrometry using the reporter molecule method.

Nian Sun1, Jiangxiao Sun, Elena N Kitova, John S Klassen.   

Abstract

The application of the reporter molecule (M(rep)) method for identifying nonspecific complexes in the ES-MS analysis of protein-ligand and DNA-ligand interactions in vitro is described. To test the reliability of the method, it was applied to the ES-MS analysis of protein-carbohydrate complexes originating from specific interactions in solution and from nonspecific interactions in the ES process. These control experiments confirm the basic assumptions underlying the M(rep) method, namely that nonspecific ligand binding is a random process, and that the ES droplet histories for specific and nonspecific complexes are distinct. The application of the M(rep) method to the ES-MS analysis of the sequential binding of the ethidium cation, a DNA intercalator, to single and double strand oligodeoxynucleotides is also described, and highlights the general utility of the method.

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Year:  2009        PMID： 19321359     DOI： 10.1016/j.jasms.2009.02.024

Source DB:  PubMed          Journal:  J Am Soc Mass Spectrom        ISSN： 1044-0305            Impact factor:   3.109


  26 in total

1.  Influence of solution and gas phase processes on protein-carbohydrate binding affinities determined by nanoelectrospray Fourier transform ion cyclotron resonance mass spectrometry.

Authors:  Weijie Wang; Elena N Kitova; John S Klassen
Journal:  Anal Chem       Date:  2003-10-01       Impact factor: 6.986

Review 2.  Investigation of intact protein complexes by mass spectrometry.

Authors:  Albert J R Heck; Robert H H Van Den Heuvel
Journal:  Mass Spectrom Rev       Date:  2004 Sep-Oct       Impact factor: 10.946

3.  Electrospray ionization mass spectrometry and ion mobility analysis of the 20S proteasome complex.

Authors:  Joseph A Loo; Beniam Berhane; Catherine S Kaddis; Kerry M Wooding; Yongming Xie; Stanley L Kaufman; Igor V Chernushevich
Journal:  J Am Soc Mass Spectrom       Date:  2005-07       Impact factor: 3.109

4.  Method for distinguishing specific from nonspecific protein-ligand complexes in nanoelectrospray ionization mass spectrometry.

Authors:  Jiangxiao Sun; Elena N Kitova; Weijie Wang; John S Klassen
Journal:  Anal Chem       Date:  2006-05-01       Impact factor: 6.986

5.  On-line dynamic titration: determination of dissociation constants for noncovalent complexes using Gaussian concentration profiles by electrospray ionization mass spectrometry.

Authors:  Petr Frycák; Kevin A Schug
Journal:  Anal Chem       Date:  2007-06-02       Impact factor: 6.986

Review 6.  Electrospray mass spectrometry to study drug-nucleic acids interactions.

Authors:  Frédéric Rosu; Edwin De Pauw; Valérie Gabelica
Journal:  Biochimie       Date:  2008-01-20       Impact factor: 4.079

7.  ESI-MS characterization of a novel pyrrole-inosine nucleoside that interacts with guanine bases.

Authors:  Sarah E Pierce; Courtney L Sherman; Janarthanan Jayawickramarajah; Candace M Lawrence; Jonathan L Sessler; Jennifer S Brodbelt
Journal:  Anal Chim Acta       Date:  2008-04-15       Impact factor: 6.558

Review 8.  Studying noncovalent protein complexes by electrospray ionization mass spectrometry.

Authors:  J A Loo
Journal:  Mass Spectrom Rev       Date:  1997 Jan-Feb       Impact factor: 10.946

9.  Ligand binding mode to duplex and triplex DNA assessed by combining electrospray tandem mass spectrometry and molecular modeling.

Authors:  Frédéric Rosu; Chi-Hung Nguyen; Edwin De Pauw; Valérie Gabelica
Journal:  J Am Soc Mass Spectrom       Date:  2007-03-28       Impact factor: 3.109

10.  Temperature-dependent cooperativity in donor-acceptor substrate binding to the human blood group glycosyltransferases.

Authors:  Glen K Shoemaker; Naoto Soya; Monica M Palcic; John S Klassen
Journal:  Glycobiology       Date:  2008-05-28       Impact factor: 4.313
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  7 in total

1.  Reliable determinations of protein-ligand interactions by direct ESI-MS measurements. Are we there yet?

Authors:  Elena N Kitova; Amr El-Hawiet; Paul D Schnier; John S Klassen
Journal:  J Am Soc Mass Spectrom       Date:  2012-01-21       Impact factor: 3.109

2.  Nonspecific interactions between proteins and charged biomolecules in electrospray ionization mass spectrometry.

Authors:  Nian Sun; Naoto Soya; Elena N Kitova; John S Klassen
Journal:  J Am Soc Mass Spectrom       Date:  2009-12-21       Impact factor: 3.109

Review 3.  Studying protein-protein affinity and immobilized ligand-protein affinity interactions using MS-based methods.

Authors:  Jeroen Kool; Niels Jonker; Hubertus Irth; Wilfried M A Niessen
Journal:  Anal Bioanal Chem       Date:  2011-07-14       Impact factor: 4.142

4.  Screening and classifying small-molecule inhibitors of amyloid formation using ion mobility spectrometry-mass spectrometry.

Authors:  Lydia M Young; Janet C Saunders; Rachel A Mahood; Charlotte H Revill; Richard J Foster; Ling-Hsien Tu; Daniel P Raleigh; Sheena E Radford; Alison E Ashcroft
Journal:  Nat Chem       Date:  2014-12-08       Impact factor: 24.427

5.  Catenation and encapsulation induce distinct reconstitutions within a dynamic library of mixed-ligand Zn4L6 cages.

Authors:  Samuel P Black; Daniel M Wood; Felix B Schwarz; Tanya K Ronson; Julian J Holstein; Artur R Stefankiewicz; Christoph A Schalley; Jeremy K M Sanders; Jonathan R Nitschke
Journal:  Chem Sci       Date:  2016-01-22       Impact factor: 9.825

Review 6.  Advances in ion mobility spectrometry-mass spectrometry reveal key insights into amyloid assembly.

Authors:  L A Woods; S E Radford; A E Ashcroft
Journal:  Biochim Biophys Acta       Date:  2012-10-11

7.  Optimization of Electrospray Ionization by Statistical Design of Experiments and Response Surface Methodology: Protein-Ligand Equilibrium Dissociation Constant Determinations.

Authors:  Liliana Pedro; Wesley C Van Voorhis; Ronald J Quinn
Journal:  J Am Soc Mass Spectrom       Date:  2016-05-25       Impact factor: 3.109
  7 in total

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