Literature DB >> 1931736

Sexing of forensic samples using PCR.

O Pascal1, D Aubert, E Gilbert, J P Moisan.   

Abstract

A rapid protocol has been established for sexing forensic samples by the Polymerase Chain Reaction method. Three sets of primer were used, two specific for Y chromosome repetitive sequences and one specific for X chromosome repetitive sequences. Detailed procedures of experiments, the controls and the applications to testing bloodstains and a vaginal swab are presented. The sensitivity of the test and problems due to contamination are discussed.

Mesh：

Substances：

Year:  1991        PMID： 1931736     DOI： 10.1007/bf01369808

Source DB:  PubMed          Journal:  Int J Legal Med        ISSN： 0937-9827            Impact factor:   2.686


  14 in total

1.  Prenatal sex determination by DNA amplification from maternal peripheral blood.

Authors:  Y M Lo; P Patel; J S Wainscoat; M Sampietro; M D Gillmer; K A Fleming
Journal:  Lancet       Date:  1989-12-09       Impact factor: 79.321

2.  A rapid method for detection of Y-chromosomal DNA from dried blood specimens by the polymerase chain reaction.

Authors:  M Witt; R P Erickson
Journal:  Hum Genet       Date:  1989-06       Impact factor: 4.132

3.  Chromosome-specific alpha satellite DNA: nucleotide sequence analysis of the 2.0 kilobasepair repeat from the human X chromosome.

Authors:  J S Waye; H F Willard
Journal:  Nucleic Acids Res       Date:  1985-04-25       Impact factor: 16.971

4.  Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.

Authors:  R K Saiki; S Scharf; F Faloona; K B Mullis; G T Horn; H A Erlich; N Arnheim
Journal:  Science       Date:  1985-12-20       Impact factor: 47.728

5.  Biopsy of human preimplantation embryos and sexing by DNA amplification.

Authors:  A H Handyside; J K Pattinson; R J Penketh; J D Delhanty; R M Winston; E G Tuddenham
Journal:  Lancet       Date:  1989-02-18       Impact factor: 79.321

6.  Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

Authors:  R K Saiki; D H Gelfand; S Stoffel; S J Scharf; R Higuchi; G T Horn; K B Mullis; H A Erlich
Journal:  Science       Date:  1988-01-29       Impact factor: 47.728

7.  Amplification of a highly polymorphic VNTR segment by the polymerase chain reaction.

Authors:  G T Horn; B Richards; K W Klinger
Journal:  Nucleic Acids Res       Date:  1989-03-11       Impact factor: 16.971

8.  An improved method for prenatal diagnosis of genetic diseases by analysis of amplified DNA sequences. Application to hemophilia A.

Authors:  S C Kogan; M Doherty; J Gitschier
Journal:  N Engl J Med       Date:  1987-10-15       Impact factor: 91.245

9.  Forensic application of DNA 'fingerprints'.

Authors:  P Gill; A J Jeffreys; D J Werrett
Journal:  Nature       Date:  1985 Dec 12-18       Impact factor: 49.962

10.  A human Y-chromosome specific repeated DNA family (DYZ1) consists of a tandem array of pentanucleotides.

Authors:  Y Nakahori; K Mitani; M Yamada; Y Nakagome
Journal:  Nucleic Acids Res       Date:  1986-10-10       Impact factor: 16.971
View more
  2 in total

1.  Sex determination of forensic samples: co-amplification and simultaneous detection of a Y-specific and an X-specific DNA sequence.

Authors:  H Pfitzinger; B Ludes; P Mangin
Journal:  Int J Legal Med       Date:  1993       Impact factor: 2.686

2.  Forensic application of a rapid and quantitative DNA sex test by amplification of the X-Y homologous gene amelogenin.

Authors:  A Mannucci; K M Sullivan; P L Ivanov; P Gill
Journal:  Int J Legal Med       Date:  1994       Impact factor: 2.686
  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.