OBJECTIVE: To compare the simple, swab 'Kudoh method' for culturing Myobacterium tuberculosis from sputum samples, to the standard Petroff digestion-decontamination procedure. The Kudoh method, which requires no centrifugation and takes only 4-5 min per sample, was also evaluated for its performance in a rural setting. METHODS: Two hospital laboratories in Caracas, Venezuela processed 314 sputum samples, in parallel, with both methods. Separately, sputum specimens were cultured with the Kudoh swab method in a field environment with minimal laboratory facilities. RESULTS: In the hospital laboratories, the sensitivity of the Kudoh swab method was comparable to that of the standard Petroff culture procedure. The swab method also performed satisfactorily in the field, improving the diagnostic sensitivity by 21% over microscopic examination alone. CONCLUSION: The Kudoh swab method is an acceptable alternative for culturing mycobacteria that is particularly suitable for rural laboratories lacking adequate infrastructure for the Petroff method.
OBJECTIVE: To compare the simple, swab 'Kudoh method' for culturing Myobacterium tuberculosis from sputum samples, to the standard Petroff digestion-decontamination procedure. The Kudoh method, which requires no centrifugation and takes only 4-5 min per sample, was also evaluated for its performance in a rural setting. METHODS: Two hospital laboratories in Caracas, Venezuela processed 314 sputum samples, in parallel, with both methods. Separately, sputum specimens were cultured with the Kudoh swab method in a field environment with minimal laboratory facilities. RESULTS: In the hospital laboratories, the sensitivity of the Kudoh swab method was comparable to that of the standard Petroff culture procedure. The swab method also performed satisfactorily in the field, improving the diagnostic sensitivity by 21% over microscopic examination alone. CONCLUSION: The Kudoh swab method is an acceptable alternative for culturing mycobacteria that is particularly suitable for rural laboratories lacking adequate infrastructure for the Petroff method.
Authors: Greta Franco-Sotomayor; Ismar A Rivera-Olivero; Margarita Leon-Benitez; Sandra E Uruchima-Campoverde; Greta Cardenas-Franco; Mercedes E Perdomo-Castro; Camilo S Cardenas-Franco; Jackeline Ortega-Vivanco; Ana S Abad-Ruiz; Jacobus H de Waard; Miguel Angel Garcia-Bereguiain Journal: J Clin Microbiol Date: 2020-03-25 Impact factor: 5.948
Authors: Eunice Atsuko Cunha; Lucilaine Ferrazoli; Lee W Riley; Paulo Cesar Basta; Michael Robert Honer; Rosalia Maia; Izaias Pereira da Costa Journal: Mem Inst Oswaldo Cruz Date: 2013-11-04 Impact factor: 2.743