Literature DB >> 1930134

Purification and partial characterization of a pyruvate oxidoreductase from the photosynthetic bacterium Rhodospirillum rubrum grown under nitrogen-fixing conditions.

E Brostedt1, S Nordlund.   

Abstract

A pyruvate oxidoreductase with the capacity to support pyruvate-dependent nitrogenase activity in vitro has been purified from the photosynthetic bacterium Rhodospirillum rubrum. The enzyme requires CoA for activity and is irreversibly inactivated by oxygen. The molecular properties and Km values for the substrates have been studied. In supporting nitrogenase activity addition of ferredoxin is required. Overall the enzyme is similar to the nif-specific pyruvate: flavodoxin oxidoreductase purified from Klebsiella pneumoniae.

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Year:  1991        PMID： 1930134      PMCID： PMC1151561          DOI： 10.1042/bj2790155

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  28 in total

1.  Light-dependent utilization of organic compounds and photoproduction of molecular hydrogen by photosynthetic bacteria; relationships with nitrogen metabolism.

Authors:  J G ORMEROD; K S ORMEROD; H GEST
Journal:  Arch Biochem Biophys       Date:  1961-09       Impact factor: 4.013

2.  Molecular cloning and sequence analysis of the structural gene of ferredoxin I from the photosynthetic bacterium Rhodobacter capsulatus.

Authors:  E Schatt; Y Jouanneau; P M Vignais
Journal:  J Bacteriol       Date:  1989-11       Impact factor: 3.490

3.  The separation of pyruvate-ferredoxin oxidoreductase from Clostridium pasteurianum into two enzymes catalyzing different reactions.

Authors:  F D Sauer; R S Bush; I L Stevenson
Journal:  Biochim Biophys Acta       Date:  1976-09-14

4.  Rapid colorimetric micromethod for the quantitation of complexed iron in biological samples.

Authors:  W W Fish
Journal:  Methods Enzymol       Date:  1988       Impact factor: 1.600

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

6.  Electron transport to nitrogenase in Klebsiella pneumoniae: purification and properties of the nifJ protein.

Authors:  D Bogusz; J Houmard; J P Aubert
Journal:  Eur J Biochem       Date:  1981-11

7.  Characterization of two soluble ferredoxins as distinct from bound iron-sulfur proteins in the photosynthetic bacterium Rhodospirillum rubrum.

Authors:  D C Yoch; D I Arnon; W V Sweeney
Journal:  J Biol Chem       Date:  1975-11-10       Impact factor: 5.157

8.  Electron transport to nitrogenase in Azotobacter chroococcum: Azotobacter flavodoxin hydroquinone as an electron donor.

Authors:  M G. Yates
Journal:  FEBS Lett       Date:  1972-10-15       Impact factor: 4.124

9.  The electron transport system in nitrogen fixation by Azotobacter. I. Azotoflavin as an electron carrier.

Authors:  J R Benemann; D C Yoch; R C Valentine; D I Arnon
Journal:  Proc Natl Acad Sci U S A       Date:  1969-11       Impact factor: 11.205

10.  Studies on the mechanism of electron transport to nitrogenase in Azotobacter vinelandii.

Authors:  J Klugkist; H Haaker; C Veeger
Journal:  Eur J Biochem       Date:  1986-02-17
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  14 in total

1.  Pyruvate ferredoxin oxidoreductase from the hyperthermophilic archaeon, Pyrococcus furiosus, functions as a CoA-dependent pyruvate decarboxylase.

Authors:  K Ma; A Hutchins; S J Sung; M W Adams
Journal:  Proc Natl Acad Sci U S A       Date:  1997-09-02       Impact factor: 11.205

Review 2.  Acetogenesis and the Wood-Ljungdahl pathway of CO(2) fixation.

Authors:  Stephen W Ragsdale; Elizabeth Pierce
Journal:  Biochim Biophys Acta       Date:  2008-08-27

3.  Isolation and analysis of the gene encoding the pyruvate-ferredoxin oxidoreductase of Desulfovibrio africanus, production of the recombinant enzyme in Escherichia coli, and effect of carboxy-terminal deletions on its stability.

Authors:  L Pieulle; V Magro; E C Hatchikian
Journal:  J Bacteriol       Date:  1997-09       Impact factor: 3.490

4.  Pyruvate: ferredoxin oxidoreductase from the sulfate-reducing Archaeoglobus fulgidus: molecular composition, catalytic properties, and sequence alignments.

Authors:  J Kunow; D Linder; R K Thauer
Journal:  Arch Microbiol       Date:  1995-01       Impact factor: 2.552

5.  Purification and properties of a nif-specific flavodoxin from the photosynthetic bacterium Rhodobacter capsulatus.

Authors:  A F Yakunin; G Gennaro; P C Hallenbeck
Journal:  J Bacteriol       Date:  1993-11       Impact factor: 3.490

6.  Characterization of a fourth type of 2-keto acid-oxidizing enzyme from a hyperthermophilic archaeon: 2-ketoglutarate ferredoxin oxidoreductase from Thermococcus litoralis.

Authors:  X Mai; M W Adams
Journal:  J Bacteriol       Date:  1996-10       Impact factor: 3.490

7.  Characterization of 2-ketoisovalerate ferredoxin oxidoreductase, a new and reversible coenzyme A-dependent enzyme involved in peptide fermentation by hyperthermophilic archaea.

Authors:  J Heider; X Mai; M W Adams
Journal:  J Bacteriol       Date:  1996-02       Impact factor: 3.490

8.  Molecular and phylogenetic characterization of pyruvate and 2-ketoisovalerate ferredoxin oxidoreductases from Pyrococcus furiosus and pyruvate ferredoxin oxidoreductase from Thermotoga maritima.

Authors:  A Kletzin; M W Adams
Journal:  J Bacteriol       Date:  1996-01       Impact factor: 3.490

9.  Properties of 2-oxoglutarate:ferredoxin oxidoreductase from Thauera aromatica and its role in enzymatic reduction of the aromatic ring.

Authors:  Edith Dörner; Matthias Boll
Journal:  J Bacteriol       Date:  2002-07       Impact factor: 3.490

10.  Identification of carboxylation enzymes and characterization of a novel four-subunit pyruvate:flavodoxin oxidoreductase from Helicobacter pylori.

Authors:  N J Hughes; P A Chalk; C L Clayton; D J Kelly
Journal:  J Bacteriol       Date:  1995-07       Impact factor: 3.490
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