CONTEXT: The protein fraction of mesenteric lymph during acute pancreatitis and other critical illness is thought to contain toxic factors. However, we do not have a complete description of the mesenteric lymph proteome during acute pancreatitis. OBJECTIVE: The aim of this study was to define the proteomic changes in mesenteric lymph during acute pancreatitis. SETTING: Animal Laboratory, University of Auckland, New Zealand. DESIGN: Mesenteric lymph was collected from sixteen male Wistar rats randomised to Group 1 (n=8) with taurocholate induced acute pancreatitis and Group 2 (n=8) sham control. The lymph was subjected to proteomic analysis using iTRAQ (Applied Biosystems, Foster City, CA, USA) and liquid chromatography-tandem mass spectrometry. RESULTS: Two hundred and forty-five proteins including 35 hypothetical proteins were identified in mesenteric lymph. Eight of the 245 proteins had a significant increase in their relative abundance in acute pancreatitis conditioned mesenteric lymph, and 7 of these were pancreatic catabolic enzymes (pancreatic amylase 2, pancreatic lipase, carboxypeptidase A2, chymotrypsinogen B, carboxypeptidase B1, cationic trypsinogen, ribonuclease 1). CONCLUSIONS: This is the first comprehensive description of the proteome of mesenteric lymph during acute pancreatitis and has demonstrated a significantly increased relative abundance of 7 secreted pancreatic catabolic enzymes in acute pancreatitis conditioned mesenteric lymph. This study provides a clear rationale for further research to investigate the efficacy of enteral protease inhibitors in the treatment of acute pancreatitis.
CONTEXT: The protein fraction of mesenteric lymph during acute pancreatitis and other critical illness is thought to contain toxic factors. However, we do not have a complete description of the mesenteric lymph proteome during acute pancreatitis. OBJECTIVE: The aim of this study was to define the proteomic changes in mesenteric lymph during acute pancreatitis. SETTING: Animal Laboratory, University of Auckland, New Zealand. DESIGN: Mesenteric lymph was collected from sixteen male Wistar rats randomised to Group 1 (n=8) with taurocholate induced acute pancreatitis and Group 2 (n=8) sham control. The lymph was subjected to proteomic analysis using iTRAQ (Applied Biosystems, Foster City, CA, USA) and liquid chromatography-tandem mass spectrometry. RESULTS: Two hundred and forty-five proteins including 35 hypothetical proteins were identified in mesenteric lymph. Eight of the 245 proteins had a significant increase in their relative abundance in acute pancreatitis conditioned mesenteric lymph, and 7 of these were pancreatic catabolic enzymes (pancreatic amylase 2, pancreatic lipase, carboxypeptidase A2, chymotrypsinogen B, carboxypeptidase B1, cationic trypsinogen, ribonuclease 1). CONCLUSIONS: This is the first comprehensive description of the proteome of mesenteric lymph during acute pancreatitis and has demonstrated a significantly increased relative abundance of 7 secreted pancreatic catabolic enzymes in acute pancreatitis conditioned mesenteric lymph. This study provides a clear rationale for further research to investigate the efficacy of enteral protease inhibitors in the treatment of acute pancreatitis.
Authors: Monika Dzieciatkowska; Max V Wohlauer; Ernest E Moore; Sagar Damle; Erik Peltz; Jeffrey Campsen; Marguerite Kelher; Christopher Silliman; Anirban Banerjee; Kirk C Hansen Journal: Shock Date: 2011-04 Impact factor: 3.454
Authors: Jerome W Breslin; Ying Yang; Joshua P Scallan; Richard S Sweat; Shaquria P Adderley; Walter L Murfee Journal: Compr Physiol Date: 2018-12-13 Impact factor: 9.090
Authors: Monika Dzieciatkowska; Angelo D'Alessandro; Ernest E Moore; Max Wohlauer; Anirban Banerjee; Christopher C Silliman; Kirk C Hansen Journal: Shock Date: 2014-12 Impact factor: 3.454
Authors: Angelo D'Alessandro; Monika Dzieciatkowska; Erik D Peltz; Ernest E Moore; Janeen R Jordan; Christopher C Silliman; Anirban Banerjee; Kirk C Hansen Journal: Shock Date: 2014-12 Impact factor: 3.454