PURPOSE: The purpose of this study was to determine the expression of MMP-12 by myofibroblasts during the healing of alkali-burned rabbit corneas (ARC), thus implicating its role in ECM remodeling. METHODS: Rabbit corneas during alkali burn were examined for MMP-12 mRNA expression by RT-PCR. Immunohistochemistry was used to determine the presence of alpha-SMA, MMP-12 protein, and macrophages. In situ hybridization was performed to identify MMP-12 mRNA expressing cells. RESULTS: RT-PCR showed that MMP-12 mRNA was expressed in the alkali-burned corneas from one week after the injury. Immunohistochemistry showed myofibroblasts positive for MMP-12 expression. In situ hybridization revealed that MMP-12 mRNA was expressed by myofibroblasts. CONCLUSION: Our results indicate that, in alkali-burned corneas, myofibroblasts express both MMP-12 mRNA and protein. We suggest that MMP-12 may disintegrate some components of the ECM released after severe alkali burn, which may be involved in the ECM remodeling.
PURPOSE: The purpose of this study was to determine the expression of MMP-12 by myofibroblasts during the healing of alkali-burned rabbit corneas (ARC), thus implicating its role in ECM remodeling. METHODS:Rabbit corneas during alkali burn were examined for MMP-12 mRNA expression by RT-PCR. Immunohistochemistry was used to determine the presence of alpha-SMA, MMP-12 protein, and macrophages. In situ hybridization was performed to identify MMP-12 mRNA expressing cells. RESULTS: RT-PCR showed that MMP-12 mRNA was expressed in the alkali-burned corneas from one week after the injury. Immunohistochemistry showed myofibroblasts positive for MMP-12 expression. In situ hybridization revealed that MMP-12 mRNA was expressed by myofibroblasts. CONCLUSION: Our results indicate that, in alkali-burned corneas, myofibroblasts express both MMP-12 mRNA and protein. We suggest that MMP-12 may disintegrate some components of the ECM released after severe alkali burn, which may be involved in the ECM remodeling.
Authors: Alan J Mouton; Osvaldo J Rivera Gonzalez; Amanda R Kaminski; Edwin T Moore; Merry L Lindsey Journal: Pharmacol Res Date: 2018-10-28 Impact factor: 7.658
Authors: Vasanthi R Sunil; Kinal N Vayas; Mingzhu Fang; Helmut Zarbl; Christopher Massa; Andrew J Gow; Jessica A Cervelli; Howard Kipen; Robert J Laumbach; Paul J Lioy; Jeffrey D Laskin; Debra L Laskin Journal: Exp Mol Pathol Date: 2016-12-13 Impact factor: 3.362
Authors: George S Karagiannis; Theofilos Poutahidis; Susan E Erdman; Richard Kirsch; Robert H Riddell; Eleftherios P Diamandis Journal: Mol Cancer Res Date: 2012-09-28 Impact factor: 5.852
Authors: Fang Bian; Flavia S A Pelegrino; Stephen C Pflugfelder; Eugene A Volpe; De-Quan Li; Cintia S de Paiva Journal: Invest Ophthalmol Vis Sci Date: 2015-07 Impact factor: 4.799
Authors: Norzana Abd Ghafar; Choy Ker-Woon; Chua Kien Hui; Yasmin Anum Mohd Yusof; Wan Zurinah Wan Ngah Journal: BMC Complement Altern Med Date: 2016-07-29 Impact factor: 3.659