Literature DB >> 23288291

Rapid detection of urinary tract infections caused by Proteus spp. using PNA-FISH.

C Almeida1, N F Azevedo, J C Bento, N Cerca, H Ramos, M J Vieira, C W Keevil.   

Abstract

We developed a fluorescence in situ hybridization (FISH) method for the rapid detection of Proteus spp. in urine, using a novel peptide nucleic acid (PNA) probe. Testing on 137 urine samples from patients with urinary tract infections has shown specificity and sensitivity values of 98 % (95 % CI, 93.2-99.7) and 100 % (95 % CI, 80,8-100), respectively, when compared with CHROMagar Orientation medium. Results indicate that PNA-FISH is a reliable alternative to traditional culture methods and can reduce the diagnosis time to approximately 2 h.

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Year:  2013        PMID： 23288291     DOI： 10.1007/s10096-012-1808-2

Source DB:  PubMed          Journal:  Eur J Clin Microbiol Infect Dis        ISSN： 0934-9723            Impact factor:   3.267


  30 in total

1.  Comparative evaluation of two commercial chromogenic media for detection and presumptive identification of urinary tract pathogens.

Authors:  C Scarparo; P Piccoli; P Ricordi; M Scagnelli
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2002-04-10       Impact factor: 3.267

2.  Use of electrochemical DNA biosensors for rapid molecular identification of uropathogens in clinical urine specimens.

Authors:  Joseph C Liao; Mitra Mastali; Vincent Gau; Marc A Suchard; Annette K Møller; David A Bruckner; Jane T Babbitt; Yang Li; Jeffrey Gornbein; Elliot M Landaw; Edward R B McCabe; Bernard M Churchill; David A Haake
Journal:  J Clin Microbiol       Date:  2006-02       Impact factor: 5.948

3.  Urinary tract infections caused by multi-drug resistant Proteus mirabilis: Risk factors and clinical outcomes.

Authors:  K Cohen-Nahum; L Saidel-Odes; K Riesenberg; F Schlaeffer; A Borer
Journal:  Infection       Date:  2009-12-07       Impact factor: 3.553

Review 4.  PNA FISH: an intelligent stain for rapid diagnosis of infectious diseases.

Authors:  Henrik Stender
Journal:  Expert Rev Mol Diagn       Date:  2003-09       Impact factor: 5.225

5.  Description of Idiomarina insulisalsae sp. nov., isolated from the soil of a sea salt evaporation pond, proposal to transfer the species of the genus Pseudidiomarina to the genus Idiomarina and emended description of the genus Idiomarina.

Authors:  Marco Taborda; André Antunes; Igor Tiago; António Veríssimo; M Fernanda Nobre; Milton S da Costa
Journal:  Syst Appl Microbiol       Date:  2009-07-22       Impact factor: 4.022

Review 6.  The etiology of urinary tract infection: traditional and emerging pathogens.

Authors:  Allan Ronald
Journal:  Am J Med       Date:  2002-07-08       Impact factor: 4.965

7.  A UK multicentre study of the antimicrobial susceptibility of bacterial pathogens causing urinary tract infection.

Authors:  D J Farrell; I Morrissey; D De Rubeis; M Robbins; D Felmingham
Journal:  J Infect       Date:  2003-02       Impact factor: 6.072

8.  Comparison of three chromogenic agar plates for isolation and identification of urinary tract pathogens.

Authors:  C Chaux; M Crepy; S Xueref; C Roure; Y Gille; A M Freydiere
Journal:  Clin Microbiol Infect       Date:  2002-10       Impact factor: 8.067

9.  Orientia tsutsugamushi stimulates an original gene expression program in monocytes: relationship with gene expression in patients with scrub typhus.

Authors:  Wiwit Tantibhedhyangkul; Thanavadee Prachason; Duangdao Waywa; Adil El Filali; Eric Ghigo; Wanna Thongnoppakhun; Didier Raoult; Yupin Suputtamongkol; Christian Capo; Chanin Limwongse; Jean-Louis Mege
Journal:  PLoS Negl Trop Dis       Date:  2011-05-17

10.  The Ribosomal Database Project (RDP-II): sequences and tools for high-throughput rRNA analysis.

Authors:  J R Cole; B Chai; R J Farris; Q Wang; S A Kulam; D M McGarrell; G M Garrity; J M Tiedje
Journal:  Nucleic Acids Res       Date:  2005-01-01       Impact factor: 16.971
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  6 in total

Review 1.  Matrix-assisted laser desorption ionization-time of flight mass spectrometry: a fundamental shift in the routine practice of clinical microbiology.

Authors:  Andrew E Clark; Erin J Kaleta; Amit Arora; Donna M Wolk
Journal:  Clin Microbiol Rev       Date:  2013-07       Impact factor: 26.132

2.  Bioinformatic Tools and Guidelines for the Design of Fluorescence In Situ Hybridization Probes.

Authors:  Helena Teixeira; Ana L Sousa; Andreia S Azevedo
Journal:  Methods Mol Biol       Date:  2021

3.  A New PNA-FISH Probe Targeting Fannyhessea vaginae.

Authors:  Lúcia G V Sousa; Joana Castro; Angela França; Carina Almeida; Christina A Muzny; Nuno Cerca
Journal:  Front Cell Infect Microbiol       Date:  2021-11-18       Impact factor: 5.293

4.  Microfluidics combined with fluorescence in situ hybridization (FISH) for Candida spp. detection.

Authors:  Violina Baranauskaite Barbosa; Célia F Rodrigues; Laura Cerqueira; João M Miranda; Nuno F Azevedo
Journal:  Front Bioeng Biotechnol       Date:  2022-09-23

5.  In vitro antibacterial, antioxidant, and cytotoxic activities of Parthenium hysterophorus and characterization of extracts by LC-MS analysis.

Authors:  Shashank Kumar; Sanjay Pandey; Abhay K Pandey
Journal:  Biomed Res Int       Date:  2014-05-07       Impact factor: 3.411

6.  Quantitative assessment of individual populations within polymicrobial biofilms.

Authors:  Susana Patrícia Lopes; Nuno Filipe Azevedo; Maria Olívia Pereira
Journal:  Sci Rep       Date:  2018-06-22       Impact factor: 4.379
  6 in total

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