Literature DB >> 19266337

Construction of gene-targeting vectors by recombineering.

Song-Choon Lee1, Wei Wang, Pentao Liu.   

Abstract

Recombineering is a technology that utilizes the efficient homologous recombination functions encoded by gamma phage to manipulate DNA in Escherichia coli. Construction of knockout vectors has been greatly facilitated by recombineering as it allows one to choose any genomic region to manipulate. We describe here an efficient recombineering-based protocol for making mouse conditional knockout targeting vectors.

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Year:  2009        PMID： 19266337      PMCID： PMC3706932          DOI： 10.1007/978-1-59745-471-1_2

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  27 in total

1.  An efficient recombination system for chromosome engineering in Escherichia coli.

Authors:  D Yu; H M Ellis; E C Lee; N A Jenkins; N G Copeland; D L Court
Journal:  Proc Natl Acad Sci U S A       Date:  2000-05-23       Impact factor: 11.205

2.  Towards genetic genome projects: genomic library screening and gene-targeting vector construction in a single step.

Authors:  Pumin Zhang; Mamei Z Li; Stephen J Elledge
Journal:  Nat Genet       Date:  2001-12-20       Impact factor: 38.330

3.  Forty years with homologous recombination.

Authors:  O Smithies
Journal:  Nat Med       Date:  2001-10       Impact factor: 53.440

Review 4.  Recombineering: a powerful new tool for mouse functional genomics.

Authors:  N G Copeland; N A Jenkins; D L Court
Journal:  Nat Rev Genet       Date:  2001-10       Impact factor: 53.242

Review 5.  Tools for targeted manipulation of the mouse genome.

Authors:  Louise van der Weyden; David J Adams; Allan Bradley
Journal:  Physiol Genomics       Date:  2002-12-03       Impact factor: 3.107

6.  A highly efficient Escherichia coli-based chromosome engineering system adapted for recombinogenic targeting and subcloning of BAC DNA.

Authors:  E C Lee; D Yu; J Martinez de Velasco; L Tessarollo; D A Swing; D L Court; N A Jenkins; N G Copeland
Journal:  Genomics       Date:  2001-04-01       Impact factor: 5.736

Review 7.  Genetic engineering using homologous recombination.

Authors:  Donald L Court; James A Sawitzke; Lynn C Thomason
Journal:  Annu Rev Genet       Date:  2002-06-11       Impact factor: 16.830

8.  A highly efficient recombineering-based method for generating conditional knockout mutations.

Authors:  Pentao Liu; Nancy A Jenkins; Neal G Copeland
Journal:  Genome Res       Date:  2003-03       Impact factor: 9.043

9.  High-throughput engineering of the mouse genome coupled with high-resolution expression analysis.

Authors:  David M Valenzuela; Andrew J Murphy; David Frendewey; Nicholas W Gale; Aris N Economides; Wojtek Auerbach; William T Poueymirou; Niels C Adams; Jose Rojas; Jason Yasenchak; Rostislav Chernomorsky; Marylene Boucher; Andrea L Elsasser; Lakeisha Esau; Jenny Zheng; Jennifer A Griffiths; Xiaorong Wang; Hong Su; Yingzi Xue; Melissa G Dominguez; Irene Noguera; Richard Torres; Lynn E Macdonald; A Francis Stewart; Thomas M DeChiara; George D Yancopoulos
Journal:  Nat Biotechnol       Date:  2003-05-05       Impact factor: 54.908

10.  A recombineering based approach for high-throughput conditional knockout targeting vector construction.

Authors:  Waiin Chan; Nina Costantino; Ruixue Li; Song Choon Lee; Qin Su; David Melvin; Donald L Court; Pentao Liu
Journal:  Nucleic Acids Res       Date:  2007-04-10       Impact factor: 16.971
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  3 in total

1.  Scarless deletion of up to seven methyl-accepting chemotaxis genes with an optimized method highlights key function of CheM in Salmonella Typhimurium.

Authors:  Stefanie Hoffmann; Christiane Schmidt; Steffi Walter; Jennifer K Bender; Roman G Gerlach
Journal:  PLoS One       Date:  2017-02-17       Impact factor: 3.240

2.  The O-Antigen Epitope Governs Susceptibility to Colistin in Salmonella enterica.

Authors:  Vito Ricci; Dexian Zhang; Christopher Teale; Laura J V Piddock
Journal:  mBio       Date:  2020-01-28       Impact factor: 7.867

3.  Lack of AcrB Efflux Function Confers Loss of Virulence on Salmonella enterica Serovar Typhimurium.

Authors:  Xuan Wang-Kan; Jessica M A Blair; Barbara Chirullo; Jonathan Betts; Roberto M La Ragione; Alasdair Ivens; Vito Ricci; Timothy J Opperman; Laura J V Piddock
Journal:  MBio       Date:  2017-07-18       Impact factor: 7.867
  3 in total

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