Literature DB >> 19260002

Shotgun mass mapping of Lactobacillus species and subspecies from caries related isolates by MALDI-MS.

Frank Schmidt1, Thomas Fiege, Hanne K Hustoft, Susanne Kneist, Bernd Thiede.   

Abstract

A taxonomical study of 90 isolates of lactobacilli isolated from soft and hard carious dentine of 70 deciduous molars is presented. The Lactobacillus strains were determined by shotgun mass mapping (SMM). This method based on MALDI-MS analysis of Lactobacillus isolates treated with trypsin followed by database comparison against a library of mass spectra derived from 20 reference strains. The SMM method allowed to discriminate different Lactobacillus subspecies. The method was used to analyse Lactobacillus isolates of unknown identity derived from carious dentine. Application of the SMM method to isolates from hard carious dentine revealed a nearly similar distribution of L. paracasei ss paracasei (29%), L. paracasei ss tolerans (32%) and L. casei ss rhamnosus (23%) as dominant subspecies. On the other hand, samples derived from soft carious dentine showed a clear bias only to L. paracasei ss paracasei (60%), whereas L. paracasei ss tolerans (14%) and L. casei ss rhamnosus (12%) were clear minorities. Compared to existent methods, SMM has unique potential for the analysis of Lactobacillus strains on subspecies level.

Entities:  

Mesh：

Substances：

Year:  2009        PMID： 19260002     DOI： 10.1002/pmic.200701028

Source DB:  PubMed          Journal:  Proteomics        ISSN： 1615-9853            Impact factor:   3.984


  9 in total

Review 1.  Mass spectrometry tools for the classification and identification of bacteria.

Authors:  Sascha Sauer; Magdalena Kliem
Journal:  Nat Rev Microbiol       Date:  2010-01       Impact factor: 60.633

2.  Determination of serotypes of Shiga toxin-producing Escherichia coli isolates by intact cell matrix-assisted laser desorption ionization-time of flight mass spectrometry.

Authors:  Axel Karger; Mario Ziller; Barbara Bettin; Birgit Mintel; Susann Schares; Lutz Geue
Journal:  Appl Environ Microbiol       Date:  2010-11-29       Impact factor: 4.792

3.  Diversity of Lactobacillus species in deep carious lesions of primary molars.

Authors:  S Kneist; F Schmidt; A Callaway; B Willershausen; S Rupf; M Wicht; B Thiede
Journal:  Eur Arch Paediatr Dent       Date:  2010-08

4.  Discrimination of different species from the genus Drosophila by intact protein profiling using matrix-assisted laser desorption ionization mass spectrometry.

Authors:  Ralph Feltens; Renate Görner; Stefan Kalkhof; Helke Gröger-Arndt; Martin von Bergen
Journal:  BMC Evol Biol       Date:  2010-04-07       Impact factor: 3.260

5.  Species determination of Culicoides biting midges via peptide profiling using matrix-assisted laser desorption ionization mass spectrometry.

Authors:  Katrin R Uhlmann; Sebastian Gibb; Stefan Kalkhof; Uriel Arroyo-Abad; Claudia Schulz; Bernd Hoffmann; Francesca Stubbins; Simon Carpenter; Martin Beer; Martin von Bergen; Ralph Feltens
Journal:  Parasit Vectors       Date:  2014-08-24       Impact factor: 3.876

6.  A Designed Experiments Approach to Optimizing MALDI-TOF MS Spectrum Processing Parameters Enhances Detection of Antibiotic Resistance in Campylobacter jejuni.

Authors:  Christian Penny; Beau Grothendick; Lin Zhang; Connie M Borror; Duane Barbano; Angela J Cornelius; Brent J Gilpin; Clifton K Fagerquist; William J Zaragoza; Michele T Jay-Russell; Albert J Lastovica; Catherine Ragimbeau; Henry-Michel Cauchie; Todd R Sandrin
Journal:  Front Microbiol       Date:  2016-05-31       Impact factor: 5.640

7.  A Randomized Controlled Open Label Crossover Trial to Study Vaginal Colonization of Orally Administered Lactobacillus Reuteri RC-14 and Rhamnosus GR-1 in Pregnant Women at High Risk for Preterm Labor.

Authors:  Enav Yefet; Raul Colodner; Merav Strauss; Yifat Gam Ze Letova; Zohar Nachum
Journal:  Nutrients       Date:  2020-04-19       Impact factor: 5.717

Review 8.  "Omic" Approaches to Bacteria and Antibiotic Resistance Identification.

Authors:  Daria Janiszewska; Małgorzata Szultka-Młyńska; Paweł Pomastowski; Bogusław Buszewski
Journal:  Int J Mol Sci       Date:  2022-08-24       Impact factor: 6.208

9.  Identification of Lactobacillus from the saliva of adult patients with caries using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

Authors:  Yifei Zhang; Yingyi Liu; Qingwei Ma; Yeqing Song; Qian Zhang; Xiaoyan Wang; Feng Chen
Journal:  PLoS One       Date:  2014-08-28       Impact factor: 3.240
  9 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.