| Literature DB >> 19252287 |
Kimihisa Itoh1, Noriko Hirata, Megumi Masuda, Shunsuke Naruto, Kazuya Murata, Keitaro Wakabayashi, Hideaki Matsuda.
Abstract
The 50% ethanolic extract (CH-ext) obtained from the unripe fruit of Citrus hassaku exhibited significant tyrosinase inhibitory activity. The CH-ext showed antioxidant activity, such as superoxide dismutase (SOD)-like activity and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity. Activity-guided fractionation of the CH-ext indicated that flavanone glycoside-rich fractions showed potent tyrosinase inhibitory activity. Further examination revealed that the tyrosinase inhibitory activity and antioxidant activity of the CH-ext were attributable to naringin and neohesperidin, respectively. The CH-ext showed inhibition of melanogenesis without any effects on cell proliferation in cultured murine B16 melanoma cells after glucosamine exposure. The topical application of the CH-ext to the dorsal skin of brownish guinea pigs showed in vivo preventive effects against UVB-induced pigmentation.Entities:
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Year: 2009 PMID： 19252287 DOI： 10.1248/bpb.32.410
Source DB: PubMed Journal: Biol Pharm Bull ISSN： 0918-6158 Impact factor: 2.233