Literature DB >> 19250916

Measurement of enzyme kinetics and inhibitor constants using enthalpy arrays.

Michael I Recht1, Frank E Torres, Dirk De Bruyker, Alan G Bell, Martin Klumpp, Richard H Bruce.   

Abstract

Enthalpy arrays enable label-free, solution-based calorimetric detection of molecular interactions in a 96-detector array format. Compared with conventional calorimetry, enthalpy arrays achieve a significant reduction of sample volume and measurement time through the combination of the small size of the detectors and ability to perform measurements in parallel. The current capabilities of the technology for studying enzyme-catalyzed reactions are demonstrated by determining the kinetic parameters for reactions with three model enzymes. In addition, the technology has been used with two classes of enzymes to determine accurate inhibitor constants for competitive inhibitors from measurements at a single inhibitor concentration.

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Year:  2009        PMID： 19250916      PMCID： PMC2722910          DOI： 10.1016/j.ab.2009.02.028

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  27 in total

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Authors:  Francisco E Torres; Peter Kuhn; Dirk De Bruyker; Alan G Bell; Michal V Wolkin; Eric Peeters; James R Williamson; Gregory B Anderson; Gregory P Schmitz; Michael I Recht; Sandra Schweizer; Lincoln G Scott; Jackson H Ho; Scott A Elrod; Peter G Schultz; Richard A Lerner; Richard H Bruce
Journal:  Proc Natl Acad Sci U S A       Date:  2004-06-21       Impact factor: 11.205

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Journal:  Arch Biochem Biophys       Date:  1968-04       Impact factor: 4.013

Review 8.  Low-molecular-weight enzyme inhibitors of microbial origin.

Authors:  H Umezawa
Journal:  Annu Rev Microbiol       Date:  1982       Impact factor: 15.500

9.  Specificities of autoinhibitory domain peptides for four protein kinases. Implications for intact cell studies of protein kinase function.

Authors:  M K Smith; R J Colbran; T R Soderling
Journal:  J Biol Chem       Date:  1990-02-05       Impact factor: 5.157

10.  Different susceptibility of protein kinases to staurosporine inhibition. Kinetic studies and molecular bases for the resistance of protein kinase CK2.

Authors:  F Meggio; A Donella Deana; M Ruzzene; A M Brunati; L Cesaro; B Guerra; T Meyer; H Mett; D Fabbro; P Furet
Journal:  Eur J Biochem       Date:  1995-11-15
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  8 in total

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Review 2.  Thermodynamic studies for drug design and screening.

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Review 3.  Higher throughput calorimetry: opportunities, approaches and challenges.

Authors:  Francisco E Torres; Michael I Recht; Joseph E Coyle; Richard H Bruce; Glyn Williams
Journal:  Curr Opin Struct Biol       Date:  2010-10-01       Impact factor: 6.809

4.  Rapid mixing of sub-microlitre drops by magnetic micro-stirring.

Authors:  Dirk De Bruyker; Michael I Recht; Ali Asgar S Bhagat; Francisco E Torres; Alan G Bell; Richard H Bruce
Journal:  Lab Chip       Date:  2011-08-15       Impact factor: 6.799

5.  Fragment-based screening for inhibitors of PDE4A using enthalpy arrays and X-ray crystallography.

Authors:  Michael I Recht; Vandana Sridhar; John Badger; Leslie Hernandez; Barbara Chie-Leon; Vicki Nienaber; Francisco E Torres
Journal:  J Biomol Screen       Date:  2012-01-05

6.  Optical calorimetry in microfluidic droplets.

Authors:  Jacob Chamoun; Ashish Pattekar; Farzaneh Afshinmanesh; Joerg Martini; Michael I Recht
Journal:  Lab Chip       Date:  2018-05-29       Impact factor: 6.799

7.  Identification and optimization of PDE10A inhibitors using fragment-based screening by nanocalorimetry and X-ray crystallography.

Authors:  Michael I Recht; Vandana Sridhar; John Badger; Pierre-Yves Bounaud; Cheyenne Logan; Barbara Chie-Leon; Vicki Nienaber; Francisco E Torres
Journal:  J Biomol Screen       Date:  2013-12-27

Review 8.  Label-free detection techniques for protein microarrays: prospects, merits and challenges.

Authors:  Sandipan Ray; Gunjan Mehta; Sanjeeva Srivastava
Journal:  Proteomics       Date:  2010-02       Impact factor: 3.984
  8 in total

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