OBJECTIVES: In this study, we analysed the expression of chitotriosidase (CHIT1) and acidic mammalian chitinase (AMCase) genes in human gastric mucosa biopsies to establish the function of the corresponding enzymes in patients with gastritis associated or not with Helicobacter pylori infection. METHODS: All 27 patients who took part in this study suffered from dyspeptic symptoms and postprandial pain, and sought to undergo gastroscopy. Antral and corpus biopsy specimens were taken to analyse stomach inflammation and detect H. pylori. RNA was extracted from antral gastric biopsies and expression of genes for CHIT1 and AMCase was analysed by quantitative real-time PCR. RESULTS: In human inflamed gastric mucosa, CHIT1 and AMCase genes were expressed on average at a very low level (approximately 10 pg), and a correlation was shown among expression of CHIT1 gene and both positivity to the H. pylori test (P = 0.016) and gastric mucosa inflammation (P = 0.026). No correlation was found among AMCase gene expression and presence of H. pylori and inflammation. CONCLUSION: In this study, we showed the presence of CHIT1 and AMCase mRNA in gastric mucosa and the correlation with the presence of H. pylori was significant only for CHIT1 but not for AMCase expression. This study has shown for the first time that CHIT1 mRNA is present in gastric mucosa and confirms the participation of such an enzyme in the human immune response to inflammation in general, and to H. pylori infection in particular.
OBJECTIVES: In this study, we analysed the expression of chitotriosidase (CHIT1) and acidic mammalian chitinase (AMCase) genes in human gastric mucosa biopsies to establish the function of the corresponding enzymes in patients with gastritis associated or not with Helicobacter pylori infection. METHODS: All 27 patients who took part in this study suffered from dyspeptic symptoms and postprandial pain, and sought to undergo gastroscopy. Antral and corpus biopsy specimens were taken to analyse stomach inflammation and detect H. pylori. RNA was extracted from antral gastric biopsies and expression of genes for CHIT1 and AMCase was analysed by quantitative real-time PCR. RESULTS: In human inflamed gastric mucosa, CHIT1 and AMCase genes were expressed on average at a very low level (approximately 10 pg), and a correlation was shown among expression of CHIT1 gene and both positivity to the H. pylori test (P = 0.016) and gastric mucosa inflammation (P = 0.026). No correlation was found among AMCase gene expression and presence of H. pylori and inflammation. CONCLUSION: In this study, we showed the presence of CHIT1 and AMCase mRNA in gastric mucosa and the correlation with the presence of H. pylori was significant only for CHIT1 but not for AMCase expression. This study has shown for the first time that CHIT1 mRNA is present in gastric mucosa and confirms the participation of such an enzyme in the human immune response to inflammation in general, and to H. pyloriinfection in particular.
Authors: Gleb Andryianau; Michal Kowalski; Michal C Piotrowicz; Adam A Rajkiewicz; Barbara Dymek; Piotr L Sklepkiewicz; Elzbieta Pluta; Filip Stefaniak; Wojciech Czestkowski; Sylwia Olejniczak; Marzena Mazur; Piotr Niedziejko; Robert Koralewski; Krzysztof Matyszewski; Mariusz Gruza; Agnieszka Zagozdzon; Magdalena Salamon; Aleksandra Rymaszewska; Mikolaj Welzer; Karolina Dzwonek; Jakub Golab; Jacek Olczak; Agnieszka Bartoszewicz; Adam Golebiowski Journal: ACS Med Chem Lett Date: 2020-04-24 Impact factor: 4.345
Authors: Intawat Nookaew; Kaisa Thorell; Kuntal Worah; Shugui Wang; Martin Lloyd Hibberd; Henrik Sjövall; Sven Pettersson; Jens Nielsen; Samuel B Lundin Journal: BMC Med Genomics Date: 2013-10-11 Impact factor: 3.063
Authors: N Manno; S Sherratt; F Boaretto; F Mejìa Coico; C Espinoza Camus; C Jara Campos; S Musumeci; A Battisti; R J Quinnell; J Mostacero León; G Vazza; M L Mostacciuolo; M G Paoletti; F H Falcone Journal: Carbohydr Polym Date: 2014-07-16 Impact factor: 9.381
Authors: J Philip Nance; Kevin M Vannella; Danielle Worth; Clément David; David Carter; Shahani Noor; Cedric Hubeau; Lori Fitz; Thomas E Lane; Thomas A Wynn; Emma H Wilson Journal: PLoS Pathog Date: 2012-11-29 Impact factor: 6.823