AIMS: Forty-nine bacteria isolated from four newly-produced waste samples of a steel industry, which had a high content of CaO, MgO, Cr and P2O5, were characterized molecularly and phenotypically by susceptibility testing against heavy metals. METHODS AND RESULTS: Phylogenetic analysis using 16S rRNA gene sequences revealed that the isolates belonged to nine genera, Pseudomonas, Micrococcus, Acinetobacter, Bacillus, Dietzia, Kocuria, Diaphorobacter, Staphylococcus and Brevibacillus. Besides, some isolates could be affiliated to species: M. luteus, Ac. junii, Ac. schindleri, B. cereus, K. marina, D. nitroreducens and Staph. warneri. The bacteria that were characterized are taxonomically diverse, and Pseudomonas and Micrococcus predominated. Fingerprinting BOX-PCR revealed high genomic heterogeneity among the isolates. Among the heavy metal compounds Zn, Ni, Pb and Cu were least toxic to the bacterial isolates, whereas Ag inhibited all isolates at 0.001 mmol l(-1). CONCLUSIONS: Heterotrophic bacteria, affiliated with several phylogentic groups, were able to colonize different wastes of a steel industry. SIGNIFICANCE AND IMPACT OF THE STUDY: This study extends our knowledge of the early colonizers bacteria populating siderurgic environments. Some of these bacteria could have potential for recycling siderurgic waste for steel production.
AIMS: Forty-nine bacteria isolated from four newly-produced waste samples of a steel industry, which had a high content of CaO, MgO, Cr and P2O5, were characterized molecularly and phenotypically by susceptibility testing against heavy metals. METHODS AND RESULTS: Phylogenetic analysis using 16S rRNA gene sequences revealed that the isolates belonged to nine genera, Pseudomonas, Micrococcus, Acinetobacter, Bacillus, Dietzia, Kocuria, Diaphorobacter, Staphylococcus and Brevibacillus. Besides, some isolates could be affiliated to species: M. luteus, Ac. junii, Ac. schindleri, B. cereus, K. marina, D. nitroreducens and Staph. warneri. The bacteria that were characterized are taxonomically diverse, and Pseudomonas and Micrococcus predominated. Fingerprinting BOX-PCR revealed high genomic heterogeneity among the isolates. Among the heavy metal compounds Zn, Ni, Pb and Cu were least toxic to the bacterial isolates, whereas Ag inhibited all isolates at 0.001 mmol l(-1). CONCLUSIONS: Heterotrophic bacteria, affiliated with several phylogentic groups, were able to colonize different wastes of a steel industry. SIGNIFICANCE AND IMPACT OF THE STUDY: This study extends our knowledge of the early colonizers bacteria populating siderurgic environments. Some of these bacteria could have potential for recycling siderurgic waste for steel production.
Authors: Mariana P Reis; Francisco A R Barbosa; Edmar Chartone-Souza; Andréa M A Nascimento Journal: Extremophiles Date: 2013-02-07 Impact factor: 2.395
Authors: Patrícia S Costa; Larissa L S Scholte; Mariana P Reis; Anderson V Chaves; Pollyanna L Oliveira; Luiza B Itabayana; Maria Luiza S Suhadolnik; Francisco A R Barbosa; Edmar Chartone-Souza; Andréa M A Nascimento Journal: PLoS One Date: 2014-04-22 Impact factor: 3.240
Authors: Dulcecleide B Freitas; Mariana P Reis; Cláudia I Lima-Bittencourt; Patrícia S Costa; Paulo S Assis; Edmar Chartone-Souza; Andréa M A Nascimento Journal: BMC Res Notes Date: 2008-10-17
Authors: Paulo Es Silva; Patrícia S Costa; Marcelo P Ávila; Maria Luíza S Suhadolnik; Mariana P Reis; Ana Paula C Salgado; Mário Fr Lima; Edmar Chartone-Souza; Andréa Ma Nascimento Journal: Springerplus Date: 2015-04-18