Literature DB >> 19235824

Detection and analysis of low-abundance cell-surface biomarkers using enzymatic amplification in microfluidic droplets.

Haakan N Joensson1, Michael L Samuels, Eric R Brouzes, Martina Medkova, Mathias Uhlén, Darren R Link, Helene Andersson-Svahn.   

Abstract

Finding the few: Cell-surface proteins are useful disease biomarkers, but current high-throughput methods are limited to detecting cells expressing more than several hundred proteins. Enzymatic amplification in microfluidic droplets (see picture) is a high-throughput method for detection and analysis of cell-surface biomarkers expressed at very low levels on individual human cells. Droplet optical labels allow concurrent analysis of several samples.

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Year:  2009        PMID： 19235824     DOI： 10.1002/anie.200804326

Source DB:  PubMed          Journal:  Angew Chem Int Ed Engl        ISSN： 1433-7851            Impact factor:   15.336


  27 in total

1.  High-resolution dose-response screening using droplet-based microfluidics.

Authors:  Oliver J Miller; Abdeslam El Harrak; Thomas Mangeat; Jean-Christophe Baret; Lucas Frenz; Bachir El Debs; Estelle Mayot; Michael L Samuels; Eamonn K Rooney; Pierre Dieu; Martin Galvan; Darren R Link; Andrew D Griffiths
Journal:  Proc Natl Acad Sci U S A       Date:  2011-12-27       Impact factor: 11.205

Review 2.  Droplet microfluidics for high-sensitivity and high-throughput detection and screening of disease biomarkers.

Authors:  Aniruddha M Kaushik; Kuangwen Hsieh; Tza-Huei Wang
Journal:  Wiley Interdiscip Rev Nanomed Nanobiotechnol       Date:  2018-05-24

3.  Parallelized ultra-high throughput microfluidic emulsifier for multiplex kinetic assays.

Authors:  Jiseok Lim; Ouriel Caen; Jérémy Vrignon; Manfred Konrad; Valérie Taly; Jean-Christophe Baret
Journal:  Biomicrofluidics       Date:  2015-05-05       Impact factor: 2.800

Review 4.  Biomarker detection for disease diagnosis using cost-effective microfluidic platforms.

Authors:  Sharma T Sanjay; Guanglei Fu; Maowei Dou; Feng Xu; Rutao Liu; Hao Qi; XiuJun Li
Journal:  Analyst       Date:  2015-11-07       Impact factor: 4.616

5.  Enhancing protease activity assay in droplet-based microfluidics using a biomolecule concentrator.

Authors:  Chia-Hung Chen; Aniruddh Sarkar; Yong-Ak Song; Miles A Miller; Sung Jae Kim; Linda G Griffith; Douglas A Lauffenburger; Jongyoon Han
Journal:  J Am Chem Soc       Date:  2011-06-20       Impact factor: 15.419

Review 6.  Droplet-based microfluidics for artificial cell generation: a brief review.

Authors:  Chiara Martino; Andrew J deMello
Journal:  Interface Focus       Date:  2016-08-06       Impact factor: 3.906

7.  Precise pooling and dispensing of microfluidic droplets towards micro- to macro-world interfacing.

Authors:  Eric Brouzes; April Carniol; Tomasz Bakowski; Helmut H Strey
Journal:  RSC Adv       Date:  2014-01-01       Impact factor: 3.361

8.  Droplet microfluidics for amplification-free genetic detection of single cells.

Authors:  Tushar D Rane; Helena C Zec; Chris Puleo; Abraham P Lee; Tza-Huei Wang
Journal:  Lab Chip       Date:  2012-07-30       Impact factor: 6.799

9.  Oil-isolated hydrogel microstructures for sensitive bioassays on-chip.

Authors:  Rathi L Srinivas; Stephen D Johnson; Patrick S Doyle
Journal:  Anal Chem       Date:  2013-11-22       Impact factor: 6.986

10.  Droplet microfluidic technology for single-cell high-throughput screening.

Authors:  Eric Brouzes; Martina Medkova; Neal Savenelli; Dave Marran; Mariusz Twardowski; J Brian Hutchison; Jonathan M Rothberg; Darren R Link; Norbert Perrimon; Michael L Samuels
Journal:  Proc Natl Acad Sci U S A       Date:  2009-07-15       Impact factor: 11.205
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