Literature DB >> 19235139

E. coli expression and purification of Fab antibody fragments.

Ka Yin Kwong1, Christoph Rader.   

Abstract

The Fab molecule was the first generated antibody fragment and still dominates basic research and clinical applications. This unit describes the E. coli expression and purification of Fab antibody fragments with and without a His tag, and is designed to yield sufficient protein for the evaluation and characterization of a panel of Fab selected from a Fab library by phage display. (c) 2009 by John Wiley & Sons, Inc.

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Year:  2009        PMID： 19235139     DOI： 10.1002/0471140864.ps0610s55

Source DB:  PubMed          Journal:  Curr Protoc Protein Sci        ISSN： 1934-3655


  13 in total

1.  Femtomolar Fab binding affinities to a protein target by alternative CDR residue co-optimization strategies without phage or cell surface display.

Authors:  Christian Votsmeier; Hanna Plittersdorf; Oliver Hesse; Andreas Scheidig; Michael Strerath; Uwe Gritzan; Klaus Pellengahr; Peter Scholz; Andrea Eicker; David Myszka; Wayne M Coco; Ulrich Haupts
Journal:  MAbs       Date:  2012-04-26       Impact factor: 5.857

2.  A novel bispecific antibody, BiSS, with potent anti-cancer activities.

Authors:  Bin Dong; Changhua Zhou; Ping He; Jing Li; Siqi Chen; Ji Miao; Qing Li; Zhong Wang
Journal:  Cancer Biol Ther       Date:  2016-02-01       Impact factor: 4.742

3.  A Sortase A Programmable Phage Display Format for Improved Panning of Fab Antibody Libraries.

Authors:  Henry D Wilson; Xiuling Li; Haiyong Peng; Christoph Rader
Journal:  J Mol Biol       Date:  2018-09-11       Impact factor: 5.469

4.  Generation, affinity maturation, and characterization of a human anti-human NKG2D monoclonal antibody with dual antagonistic and agonistic activity.

Authors:  Ka Yin Kwong; Sivasubramanian Baskar; Hua Zhang; Crystal L Mackall; Christoph Rader
Journal:  J Mol Biol       Date:  2008-09-16       Impact factor: 5.469

5.  Mining Naïve Rabbit Antibody Repertoires by Phage Display for Monoclonal Antibodies of Therapeutic Utility.

Authors:  Haiyong Peng; Thomas Nerreter; Jing Chang; Junpeng Qi; Xiuling Li; Pabalu Karunadharma; Gustavo J Martinez; Mohammad Fallahi; Jo Soden; Jim Freeth; Roger R Beerli; Ulf Grawunder; Michael Hudecek; Christoph Rader
Journal:  J Mol Biol       Date:  2017-08-14       Impact factor: 5.469

6.  Generation and characterization of a chimeric rabbit/human Fab for co-crystallization of HIV-1 Rev.

Authors:  Stephen J Stahl; Norman R Watts; Christoph Rader; Michael A DiMattia; Rose G Mage; Ira Palmer; Joshua D Kaufman; Jonathan M Grimes; David I Stuart; Alasdair C Steven; Paul T Wingfield
Journal:  J Mol Biol       Date:  2010-02-04       Impact factor: 5.469

7.  Molecularly defined antibody conjugation through a selenocysteine interface.

Authors:  Thomas Hofer; Lauren R Skeffington; Colby M Chapman; Christoph Rader
Journal:  Biochemistry       Date:  2009-12-22       Impact factor: 3.162

8.  A HER2 bispecific antibody can be efficiently expressed in Escherichia coli with potent cytotoxicity.

Authors:  Limin Lin; Li Li; Changhua Zhou; Jing Li; Jiayu Liu; Rui Shu; Bin Dong; Qing Li; Zhong Wang
Journal:  Oncol Lett       Date:  2018-05-11       Impact factor: 2.967

9.  Therapeutic potential and challenges of targeting receptor tyrosine kinase ROR1 with monoclonal antibodies in B-cell malignancies.

Authors:  Jiahui Yang; Sivasubramanian Baskar; Ka Yin Kwong; Michael G Kennedy; Adrian Wiestner; Christoph Rader
Journal:  PLoS One       Date:  2011-06-15       Impact factor: 3.240

10.  Selection of apoptotic cell specific human antibodies from adult bone marrow.

Authors:  Caroline Grönwall; Edgar D Charles; Lynn B Dustin; Christoph Rader; Gregg J Silverman
Journal:  PLoS One       Date:  2014-04-23       Impact factor: 3.240
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