PURPOSE: This study examined the osteogenic phenotypes and mineralization of cultured human dental papilla-derived cells. MATERIALS AND METHODS: Dental papillae were harvested from mandibles during surgical extraction of lower impacted third molars from 3 patients aged 13 to 15 years. The dental papilla-derived cells were introduced into the cell culture. After passage 3, the dental papilla-derived cells were further cultured for 42 days in an osteogenic inductive culture medium containing dexamethasone, ascorbic acid, and beta-glycerophosphate. We examined the histochemical detection of alkaline phosphatase (ALP), the reverse transcriptase-polymerase chain reaction (RT-PCR) analysis for ALP and osteocalcin, and von Kossa staining in the dental papilla-derived cells. RESULTS: It was observed that ALP was strongly expressed in the earlier stage of osteoblastic differentiation, whereas osteocalcin was mainly expressed and secreted into the medium at the later stage. Von Kossa-positive mineralization nodules were first observed on day 14, which increased in number during the entire culture period. CONCLUSIONS: These results suggest that dental papilla-derived cell have osteogenic potential and could be used as an additional source of cells for bone tissue engineering.
PURPOSE: This study examined the osteogenic phenotypes and mineralization of cultured human dental papilla-derived cells. MATERIALS AND METHODS: Dental papillae were harvested from mandibles during surgical extraction of lower impacted third molars from 3 patients aged 13 to 15 years. The dental papilla-derived cells were introduced into the cell culture. After passage 3, the dental papilla-derived cells were further cultured for 42 days in an osteogenic inductive culture medium containing dexamethasone, ascorbic acid, and beta-glycerophosphate. We examined the histochemical detection of alkaline phosphatase (ALP), the reverse transcriptase-polymerase chain reaction (RT-PCR) analysis for ALP and osteocalcin, and von Kossa staining in the dental papilla-derived cells. RESULTS: It was observed that ALP was strongly expressed in the earlier stage of osteoblastic differentiation, whereas osteocalcin was mainly expressed and secreted into the medium at the later stage. Von Kossa-positive mineralization nodules were first observed on day 14, which increased in number during the entire culture period. CONCLUSIONS: These results suggest that dental papilla-derived cell have osteogenic potential and could be used as an additional source of cells for bone tissue engineering.
Authors: M Paschalidou; E Athanasiadou; K Arapostathis; N Kotsanos; P T Koidis; Athina Bakopoulou; Anna Theocharidou Journal: Clin Oral Investig Date: 2019-05-08 Impact factor: 3.573
Authors: Maximilian E H Wagner; Andreas Kampmann; Kathrin Schumann-Moor; Nils-Claudius Gellrich; Frank Tavassol; Friederike Schmeltekop; Martin Rücker; Martin Lanzer; Thomas Gander; Harald Essig; Paul Schumann Journal: Hypertens Res Date: 2020-08-11 Impact factor: 3.872