AIMS: The aim of the work was to evaluate the circulation of the viruses and to determine a correlation between faecal indicators and viruses. METHODS AND RESULTS: Raw wastewater and effluent samples were collected from three wastewater treatment plants, during three sampling periods, and analysed, using cultural and molecular methods, to determine bacteria and virus presence. The results show a removal of bacterial indicators, but a limited reduction of the phages. The viral analysis displays the circulation of cultivable enteroviruses and differences in the seasonal-geographical distribution. Hepatitis A virus was found with only two genotypes: IA-IB. Rotavirus was present in 11.11%, 24.14%, 2.78% of the samples in the 1st, 2nd and 3rd sampling periods; Astrovirus in 33.33%, 6.9%, 25%; Adenovirus in 7.41%, 3.45%, 2.78%; Norovirus in 7.41%, 10.34%, 5.56% respectively. Adenovirus was never identified in plants B and C as Rotavirus in plant C. CONCLUSIONS: The presence of faecal indicators was not predictive of the enteric virus presence, whereas a different circulation of Enteroviruses was found in the wastewater treatment plants. SIGNIFICANCE AND IMPACT OF THE STUDY: The study shows the importance and the usefulness of molecular methods to evaluate the virus circulation and the genetic variability of Enteroviruses.
AIMS: The aim of the work was to evaluate the circulation of the viruses and to determine a correlation between faecal indicators and viruses. METHODS AND RESULTS: Raw wastewater and effluent samples were collected from three wastewater treatment plants, during three sampling periods, and analysed, using cultural and molecular methods, to determine bacteria and virus presence. The results show a removal of bacterial indicators, but a limited reduction of the phages. The viral analysis displays the circulation of cultivable enteroviruses and differences in the seasonal-geographical distribution. Hepatitis A virus was found with only two genotypes: IA-IB. Rotavirus was present in 11.11%, 24.14%, 2.78% of the samples in the 1st, 2nd and 3rd sampling periods; Astrovirus in 33.33%, 6.9%, 25%; Adenovirus in 7.41%, 3.45%, 2.78%; Norovirus in 7.41%, 10.34%, 5.56% respectively. Adenovirus was never identified in plants B and C as Rotavirus in plant C. CONCLUSIONS: The presence of faecal indicators was not predictive of the enteric virus presence, whereas a different circulation of Enteroviruses was found in the wastewater treatment plants. SIGNIFICANCE AND IMPACT OF THE STUDY: The study shows the importance and the usefulness of molecular methods to evaluate the virus circulation and the genetic variability of Enteroviruses.
Authors: P Kokkinos; G Mandilara; A Nikolaidou; A Velegraki; P Theodoratos; D Kampa; A Blougoura; A Christopoulou; E Smeti; G Kamizoulis; A Vantarakis; A Mavridou Journal: Environ Sci Pollut Res Int Date: 2015-07-09 Impact factor: 4.223
Authors: A Battistone; G Buttinelli; S Fiore; C Amato; P Bonomo; A M Patti; A Vulcano; M Barbi; S Binda; L Pellegrinelli; M L Tanzi; P Affanni; P Castiglia; C Germinario; P Mercurio; A Cicala; M Triassi; F Pennino; L Fiore Journal: Appl Environ Microbiol Date: 2014-08 Impact factor: 4.792
Authors: Franco M Ruggeri; Paolo Bonomo; Giovanni Ianiro; Andrea Battistone; Roberto Delogu; Cinzia Germinario; Maria Chironna; Maria Triassi; Rosalba Campagnuolo; Antonella Cicala; Giovanni M Giammanco; Paolo Castiglia; Caterina Serra; Andrea Gaggioli; Lucia Fiore Journal: Appl Environ Microbiol Date: 2014-10-24 Impact factor: 4.792
Authors: Francesca Pennino; Antonio Nardone; Paolo Montuori; Sara Aurino; Ida Torre; Andrea Battistone; Roberto Delogu; Gabriele Buttinelli; Stefano Fiore; Concetta Amato; Maria Triassi Journal: Food Environ Virol Date: 2017-12-16 Impact factor: 2.778
Authors: Andrêssa Silvino Ferreira Assis; Tulio Machado Fumian; Marize Pereira Miagostovich; Betânia Paiva Drumond; Maria Luzia da Rosa E Silva Journal: Environ Sci Pollut Res Int Date: 2018-04-09 Impact factor: 4.223
Authors: Hugo Ramiro Poma; Verónica Beatriz Rajal; María Dolores Blanco Fernández; Patricia Angélica Barril; Miguel Oscar Giordano; Gisela Masachessi; Laura Cecilia Martínez; María Beatriz Isa; María Cecilia Freire; Gabriela López Riviello; Daniel Cisterna; Silvia Viviana Nates; Viviana Andrea Mbayed Journal: Environ Monit Assess Date: 2012-07-06 Impact factor: 2.513