Literature DB >> 19209756

PCR versus ELISA in diagnosis of human toxoplasmosis in Jeddah, Saudi Arabia.

Abdulkader M D Tonkal1.   

Abstract

In this study, polymerase chain reaction (PCR) was compared with detection of specific antibodies to Toxoplasma by ELISA. A total of 70 blood samples from patients with different clinical features were tested, 43/70 (61.4%) were serologically Toxoplasma-IgG positive meanwhile only 16/43 (37.2%) were approved to be positive by PCR. A total of 22 samples (31.6%) were positive by PCR, but only 16 of them were positive by ELISA. So, Tox-IgG indicated catching Toxoplasma infection but not enough with immune modulation to determine acute case, while PCR besides, being valuable in diagnosis showed Toxoplasma parasitemia.

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Year:  2008        PMID： 19209756

Source DB:  PubMed          Journal:  J Egypt Soc Parasitol        ISSN： 1110-0583


  8 in total

1.  Seroprevalence and risk factors of Toxoplasma gondii infection among pregnant women in south western, Saudi Arabia.

Authors:  Mona A Almushait; Saad M Bin Dajem; Nahla M Elsherbiny; Mamdoh A Eskandar; Tarik A Al Azraqi; Laila M Makhlouf
Journal:  J Parasit Dis       Date:  2012-11-02

Review 2.  DNA Amplification Techniques for the Detection of Toxoplasma gondii Tissue Cysts in Meat Producing Animals: A Narrative Review Article.

Authors:  Farooq Riaz; Imran Rashid; Haroon Akbar; Wasim Shehzad; Saher Islam; Amna Arshad Bajwa; Khalid Saeed; Kamran Ashraf
Journal:  Iran J Parasitol       Date:  2016 Oct-Dec       Impact factor: 1.012

3.  Knowledge and Attitude Regarding Toxoplasmosis among Jazan University Female Students.

Authors:  Mohamed S Mahfouz; Mona Elmahdy; Ahmed Bahri; Yara Mohammed Mobarki; Atheer Ali Altalhi; Norah Abdullah Barkat; Halimah Abdullah Al-Essa; Asmaa Hussein Ageely; Nesrin Ahmed Faqeeh; Nuha Abker Areeshi; Salma Mohammed Al-Hassan
Journal:  Saudi J Med Med Sci       Date:  2018-12-14

4.  Serological and molecular approaches in clinical diagnosis of ocular toxoplasmosis in Iran.

Authors:  Vafa Saber; Seyyed Javad Seyyed Tabaei; Seyyed Ali Tabatabaei; Mohammad Soleimani; Ali Haghighi
Journal:  Med J Islam Repub Iran       Date:  2019-08-12

5.  Seroepidemiology of Toxoplasma gondii amongst Pregnant Women in Jazan Province, Saudi Arabia.

Authors:  Hussein Aqeely; Eman K El-Gayar; Darakhshan Perveen Khan; Abdullah Najmi; Ayesha Alvi; Ibrahim Bani; Mohamed Salih Mahfouz; Saif Elden Abdalla; Ibrahim M Elhassan
Journal:  J Trop Med       Date:  2014-11-13

6.  Detection of Toxoplasma gondii in Diabetic Patients Using the Nested PCR Assay via RE and B1 Genes.

Authors:  Mohammad Mousavi; Ramin Saravani; Mohammad Jafari Modrek; Mahnaz Shahrakipour; Sina Sekandarpour
Journal:  Jundishapur J Microbiol       Date:  2016-02-09       Impact factor: 0.747

7.  Toxoplasmosis preventive behavior and related knowledge among Saudi pregnant women: an exploratory study.

Authors:  Tarek Tawfik Amin; Mohamed Nabil Al Ali; Ahmed Abdulmohsen Alrashid; Amena Ahmed Al-Agnam; Amina Abdullah Al Sultan
Journal:  Glob J Health Sci       Date:  2013-06-21

8.  Detection of Toxoplasma gondii DNA by PCR in blood samples collected from pregnant Saudi women from the Aseer region, Saudi Arabia.

Authors:  Saad M Bin Dajem; Mona A Almushait
Journal:  Ann Saudi Med       Date:  2012 Sep-Oct       Impact factor: 1.526
  8 in total

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