| Literature DB >> 1920636 |
Y Nakanishi1, Y Masamune, N Kobayashi.
Abstract
cis-acting elements for the transcription of human immunodeficiency virus type 1 DNA were analyzed in cell-free transcription and DNA transfection assays. Besides previously identified cis elements, a region adjacent to the enhancer element was found to regulate transcription in both assays. Loss of this region caused 4.3- and 1.6-fold transcription inhibition in a transfection assay with a T-cell line, MOLT-4, and a monocyte line, U937, respectively, whereas the same region appeared to function negatively with other T-cell lines, MT-4 and Jurkat. These results suggest that this novel cis element regulates the transcription of proviral DNA in a cell-type-specific manner.Entities:
Mesh:
Substances:
Year: 1991 PMID: 1920636 PMCID: PMC250349
Source DB: PubMed Journal: J Virol ISSN: 0022-538X Impact factor: 5.103