| Literature DB >> 19177179 |
Thomas O Metz1, Qibin Zhang, Jason S Page, Yufeng Shen, Stephen J Callister, Jon M Jacobs, Richard D Smith.
Abstract
The future utility of liquid chromatography-mass spectrometry (LC-MS) in metabolic profiling and metabolomic studies for biomarker discover will be discussed, beginning with a brief description of the evolution of metabolomics and the utilization of the three most popular analytical platforms in such studies: NMR, GC-MS, and LC-MS. Emphasis is placed on recent developments in high-efficiency LC separations, sensitive electrospray ionization approaches, and the benefits to incorporating both in LC-MS-based approaches. The advantages and disadvantages of various quantitative approaches are reviewed, followed by the current LC-MS-based tools available for candidate biomarker characterization and identification. Finally, a brief prediction on the future path of LC-MS-based methods in metabolic profiling and metabolomic studies is given.Year: 2007 PMID： 19177179 PMCID： PMC2631423 DOI： 10.2217/17520363.1.1.159
Source DB: PubMed Journal: Biomark Med ISSN： 1752-0363 Impact factor: 2.851