Literature DB >> 1916297

Production of functional rat HMG1 protein in Escherichia coli.

M E Bianchi1.   

Abstract

High-mobility group-1 protein (HMG1) was produced in Escherichia coli under the control of the T7 promoter/T7 RNA polymerase system. The protein can be produced and purified with yields similar to those obtained from animal tissues. HMG1 purified from E. coli is homogeneous and capable of selectively binding cruciform DNA, indicating that post-translational processing of vertebrate HMG1 is not necessary for its DNA-binding ability.

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Year:  1991        PMID： 1916297     DOI： 10.1016/0378-1119(91)90261-9

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  12 in total

1.  DNA binding by single HMG box model proteins.

Authors:  H Xin; S Taudte; N R Kallenbach; M P Limbach; R S Zitomer
Journal:  Nucleic Acids Res       Date:  2000-10-15       Impact factor: 16.971

2.  High-mobility-group 1 protein mediates DNA bending as determined by ring closures.

Authors:  P M Pil; C S Chow; S J Lippard
Journal:  Proc Natl Acad Sci U S A       Date:  1993-10-15       Impact factor: 11.205

3.  Purification and properties of a cruciform DNA binding protein from Ustilago maydis.

Authors:  H Kotani; E B Kmiec; W K Holloman
Journal:  Chromosoma       Date:  1993-05       Impact factor: 4.316

4.  Alanine mutagenesis of high-mobility-group-protein-1 box B (HMG1-B).

Authors:  S Taudte; H Xin; N R Kallenbach
Journal:  Biochem J       Date:  2000-05-01       Impact factor: 3.857

5.  High-mobility-group proteins and cancer--an emerging link.

Authors:  V Wunderlich; M Böttger
Journal:  J Cancer Res Clin Oncol       Date:  1997       Impact factor: 4.553

6.  Molecular cloning, nucleotide sequence, and expression of a carboxypeptidase-encoding gene from the archaebacterium Sulfolobus solfataricus.

Authors:  S Colombo; G Toietta; L Zecca; M Vanoni; P Tortora
Journal:  J Bacteriol       Date:  1995-10       Impact factor: 3.490

7.  HMG-domain proteins specifically inhibit the repair of the major DNA adduct of the anticancer drug cisplatin by human excision nuclease.

Authors:  J C Huang; D B Zamble; J T Reardon; S J Lippard; A Sancar
Journal:  Proc Natl Acad Sci U S A       Date:  1994-10-25       Impact factor: 11.205

8.  Structure and specificity of T cell receptors expressed by potentially pathogenic anti-DNA autoantibody-inducing T cells in human lupus.

Authors:  A Desai-Mehta; C Mao; S Rajagopalan; T Robinson; S K Datta
Journal:  J Clin Invest       Date:  1995-02       Impact factor: 14.808

9.  SRY, like HMG1, recognizes sharp angles in DNA.

Authors:  S Ferrari; V R Harley; A Pontiggia; P N Goodfellow; R Lovell-Badge; M E Bianchi
Journal:  EMBO J       Date:  1992-12       Impact factor: 11.598

10.  Amino acid residues 201-205 in C-terminal acidic tail region plays a crucial role in antibacterial activity of HMGB1.

Authors:  Wei Gong; Yuan Li; Fan Chao; Gang Huang; Fengtian He
Journal:  J Biomed Sci       Date:  2009-09-14       Impact factor: 8.410
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