Literature DB >> 19160512

Efficient microRNA capture and bar-coding via enzymatic oligonucleotide adenylation.

Francois Vigneault1, A Michael Sismour, George M Church.   

Abstract

Here we report a highly efficient and simplified strategy to preadenylate bar-coded oligonucleotides designed for microRNA (miRNA) capture and multiplex analysis. Using this approach, we enzymatically preadenylated bar-coded oligonucleotides with high efficiency when compared to the chemical method currently used by miRNA investigators. As a case study, we used these oligonucleotides in an ATP-independent ligation to miRNAs, suggesting the utility of our method in end-capture protocols and high-throughput sequencing applications.

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Year:  2008        PMID： 19160512     DOI： 10.1038/nmeth.1244

Source DB:  PubMed          Journal:  Nat Methods        ISSN： 1548-7091            Impact factor:   28.547


  26 in total

1.  Deep sequencing of microRNA precursors reveals extensive 3' end modification.

Authors:  Martin A Newman; Vidya Mani; Scott M Hammond
Journal:  RNA       Date:  2011-08-17       Impact factor: 4.942

2.  RNA-ligase-dependent biases in miRNA representation in deep-sequenced small RNA cDNA libraries.

Authors:  Markus Hafner; Neil Renwick; Miguel Brown; Aleksandra Mihailović; Daniel Holoch; Carolina Lin; John T G Pena; Jeffrey D Nusbaum; Pavel Morozov; Janos Ludwig; Tolulope Ojo; Shujun Luo; Gary Schroth; Thomas Tuschl
Journal:  RNA       Date:  2011-07-20       Impact factor: 4.942

3.  Barcoding bias in high-throughput multiplex sequencing of miRNA.

Authors:  Shahar Alon; Francois Vigneault; Seda Eminaga; Danos C Christodoulou; Jonathan G Seidman; George M Church; Eli Eisenberg
Journal:  Genome Res       Date:  2011-07-12       Impact factor: 9.043

4.  High-throughput multiplex sequencing of miRNA.

Authors:  Francois Vigneault; Dmitry Ter-Ovanesyan; Shahar Alon; Seda Eminaga; Danos C Christodoulou; J G Seidman; Eli Eisenberg; George M Church
Journal:  Curr Protoc Hum Genet       Date:  2012-04

Review 5.  Library construction for next-generation sequencing: overviews and challenges.

Authors:  Steven R Head; H Kiyomi Komori; Sarah A LaMere; Thomas Whisenant; Filip Van Nieuwerburgh; Daniel R Salomon; Phillip Ordoukhanian
Journal:  Biotechniques       Date:  2014-02-01       Impact factor: 1.993

6.  Quantification of microRNA expression with next-generation sequencing.

Authors:  Seda Eminaga; Danos C Christodoulou; Francois Vigneault; George M Church; J G Seidman
Journal:  Curr Protoc Mol Biol       Date:  2013-07

Review 7.  MicroRNAs in human cancer.

Authors:  Thalia A Farazi; Jessica I Hoell; Pavel Morozov; Thomas Tuschl
Journal:  Adv Exp Med Biol       Date:  2013       Impact factor: 2.622

8.  Recognition of 5' triphosphate by RIG-I helicase requires short blunt double-stranded RNA as contained in panhandle of negative-strand virus.

Authors:  Martin Schlee; Andreas Roth; Veit Hornung; Cristina Amparo Hagmann; Vera Wimmenauer; Winfried Barchet; Christoph Coch; Markus Janke; Aleksandra Mihailovic; Greg Wardle; Stefan Juranek; Hiroki Kato; Taro Kawai; Hendrik Poeck; Katherine A Fitzgerald; Osamu Takeuchi; Shizuo Akira; Thomas Tuschl; Eicke Latz; Janos Ludwig; Gunther Hartmann
Journal:  Immunity       Date:  2009-07-02       Impact factor: 31.745

9.  Capture, amplification, and global profiling of microRNAs from low quantities of whole cell lysate.

Authors:  Nayi Wang; Jijun Cheng; Rong Fan; Jun Lu
Journal:  Analyst       Date:  2017-08-21       Impact factor: 4.616

10.  Barcoded cDNA library preparation for small RNA profiling by next-generation sequencing.

Authors:  Markus Hafner; Neil Renwick; Thalia A Farazi; Aleksandra Mihailović; John T G Pena; Thomas Tuschl
Journal:  Methods       Date:  2012-08-07       Impact factor: 3.608
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