Literature DB >> 23821442

Quantification of microRNA expression with next-generation sequencing.

Seda Eminaga1, Danos C Christodoulou, Francois Vigneault, George M Church, J G Seidman.   

Abstract

Rapid advancement of next-generation sequencing technologies has made it possible to study expression profiles of microRNAs (miRNAs) comprehensively and efficiently. Multiplexing miRNA libraries by barcoding can significantly reduce sequencing cost per sample without compromising library quality. This unit provides a step-by-step protocol for isolating miRNAs and constructing multiplexed miRNA libraries. Also described is a custom computational pipeline for analyzing the multiplexed miRNA library sequencing reads generated by Illumina-based technology.
© 2013 by John Wiley & Sons, Inc.

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Year:  2013        PMID： 23821442      PMCID： PMC4138881          DOI： 10.1002/0471142727.mb0417s103

Source DB:  PubMed          Journal:  Curr Protoc Mol Biol        ISSN： 1934-3647


  16 in total

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