Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 An orthogonal proteomic-genomic screen identifies AIM2 as a cytoplasmic DNA sensor for the inflammasome.

Literature DB >> 19158679

An orthogonal proteomic-genomic screen identifies AIM2 as a cytoplasmic DNA sensor for the inflammasome.

Tilmann Bürckstümmer¹, Christoph Baumann, Stephan Blüml, Evelyn Dixit, Gerhard Dürnberger, Hannah Jahn, Melanie Planyavsky, Martin Bilban, Jacques Colinge, Keiryn L Bennett, Giulio Superti-Furga.

Abstract

Cytoplasmic DNA triggers activation of the innate immune system. Although 'downstream' signaling components have been characterized, the DNA-sensing components remain elusive. Here we present a systematic proteomics screen for proteins that associate with DNA, 'crossed' to a screen for transcripts induced by interferon-beta, which identified AIM2 as a candidate cytoplasmic DNA sensor. AIM2 showed specificity for double-stranded DNA. It also recruited the inflammasome adaptor ASC and localized to ASC 'speckles'. A decrease in AIM2 expression produced by RNA-mediated interference impaired DNA-induced maturation of interleukin 1beta in THP-1 human monocytic cells, which indicated that endogenous AIM2 is required for DNA recognition. Reconstitution of unresponsive HEK293 cells with AIM2, ASC, caspase-1 and interleukin 1beta showed that AIM2 was sufficient for inflammasome activation. Our data suggest that AIM2 is a cytoplasmic DNA sensor for the inflammasome.

Entities: Gene Species

Mesh：

Substances：

Year: 2009 PMID： 19158679 DOI： 10.1038/ni.1702

Source DB: PubMed Journal: Nat Immunol ISSN： 1529-2908 Impact factor: 25.606

28 in total

1. The HIN domain of IFI-200 proteins consists of two OB folds.

Authors: Mario Albrecht; Divaker Choubey; Thomas Lengauer
Journal: Biochem Biophys Res Commun Date: 2005-02-18 Impact factor: 3.575

2. A Shope Fibroma virus PYRIN-only protein modulates the host immune response.

Authors: Andrea Dorfleutner; Siera J Talbott; Nicole B Bryan; Kristin N Funya; Stephanie L Rellick; John C Reed; Xianglin Shi; Yon Rojanasakul; Daniel C Flynn; Christian Stehlik
Journal: Virus Genes Date: 2007-08-04 Impact factor: 2.332

Review 3. Intracellular NOD-like receptors in host defense and disease.

Authors: Thirumala-Devi Kanneganti; Mohamed Lamkanfi; Gabriel Núñez
Journal: Immunity Date: 2007-10 Impact factor: 31.745

Review 4. Innate recognition of viruses.

Authors: Andreas Pichlmair; Caetano Reis e Sousa
Journal: Immunity Date: 2007-09 Impact factor: 31.745

5. Interferon-inducible gene expression signature in peripheral blood cells of patients with severe lupus.

Authors: Emily C Baechler; Franak M Batliwalla; George Karypis; Patrick M Gaffney; Ward A Ortmann; Karl J Espe; Katherine B Shark; William J Grande; Karis M Hughes; Vivek Kapur; Peter K Gregersen; Timothy W Behrens
Journal: Proc Natl Acad Sci U S A Date: 2003-02-25 Impact factor: 11.205

6. Role of charged and hydrophobic residues in the oligomerization of the PYRIN domain of ASC.

Authors: Mie Moriya; Shunichiro Taniguchi; Peter Wu; Edvards Liepinsh; Gottfried Otting; Junji Sagara
Journal: Biochemistry Date: 2005-01-18 Impact factor: 3.162

7. Gene-targeted mice lacking the Trex1 (DNase III) 3'-->5' DNA exonuclease develop inflammatory myocarditis.

Authors: Masashi Morita; Gordon Stamp; Peter Robins; Anna Dulic; Ian Rosewell; Geza Hrivnak; Graham Daly; Tomas Lindahl; Deborah E Barnes
Journal: Mol Cell Biol Date: 2004-08 Impact factor: 4.272

8. Silica crystals and aluminum salts activate the NALP3 inflammasome through phagosomal destabilization.

Authors: Veit Hornung; Franz Bauernfeind; Annett Halle; Eivind O Samstad; Hajime Kono; Kenneth L Rock; Katherine A Fitzgerald; Eicke Latz
Journal: Nat Immunol Date: 2008-07-11 Impact factor: 25.606

9. A novel role of the interferon-inducible protein IFI16 as inducer of proinflammatory molecules in endothelial cells.

Authors: Patrizia Caposio; Francesca Gugliesi; Claudia Zannetti; Simone Sponza; Michele Mondini; Enzo Medico; John Hiscott; Howard A Young; Giorgio Gribaudo; Marisa Gariglio; Santo Landolfo
Journal: J Biol Chem Date: 2007-08-14 Impact factor: 5.157

10. Trex1 prevents cell-intrinsic initiation of autoimmunity.

Authors: Daniel B Stetson; Joan S Ko; Thierry Heidmann; Ruslan Medzhitov
Journal: Cell Date: 2008-08-22 Impact factor: 41.582

486 in total

Review 1. The immunology of acute stroke.

Authors: Ángel Chamorro; Andreas Meisel; Anna M Planas; Xabier Urra; Diederik van de Beek; Roland Veltkamp
Journal: Nat Rev Neurol Date: 2012-06-05 Impact factor: 42.937

2. Absent in melanoma 2 is required for innate immune recognition of Francisella tularensis.

Authors: Jonathan W Jones; Nobuhiko Kayagaki; Petr Broz; Thomas Henry; Kim Newton; Karen O'Rourke; Salina Chan; Jennifer Dong; Yan Qu; Meron Roose-Girma; Vishva M Dixit; Denise M Monack
Journal: Proc Natl Acad Sci U S A Date: 2010-05-10 Impact factor: 11.205

3. The cytosolic nucleic acid sensor LRRFIP1 mediates the production of type I interferon via a beta-catenin-dependent pathway.

Authors: Pengyuan Yang; Huazhang An; Xingguang Liu; Mingyue Wen; Yuanyuan Zheng; Yaocheng Rui; Xuetao Cao
Journal: Nat Immunol Date: 2010-05-09 Impact factor: 25.606

4. Deletion of ripA alleviates suppression of the inflammasome and MAPK by Francisella tularensis.

Authors: Max Tze-Han Huang; Brittany L Mortensen; Debra J Taxman; Robin R Craven; Sharon Taft-Benz; Todd M Kijek; James R Fuller; Beckley K Davis; Irving Coy Allen; Willie June Brickey; Denis Gris; Haitao Wen; Thomas H Kawula; Jenny Pan-Yun Ting
Journal: J Immunol Date: 2010-10-04 Impact factor: 5.422