Literature DB >> 19156778

A pyrrolysine analogue for protein click chemistry.

Tomasz Fekner1, Xin Li, Marianne M Lee, Michael K Chan.   

Abstract

Ignoring the STOP sign: A pyrrolysine analogue bearing a terminal alkyne was site-specifically incorporated into recombinant calmodulin (CaM) through a UAG codon. The resulting protein was labeled with an azide-containing dye using a copper(I)-catalyzed click reaction. Subsequent application of an orthogonal cysteine tagging method yielded a CaM labeled with two distinct fluorophores that enabled its study by FRET spectroscopy.

Entities:  

Mesh：

Substances：

Year:  2009        PMID： 19156778     DOI： 10.1002/anie.200805420

Source DB:  PubMed          Journal:  Angew Chem Int Ed Engl        ISSN： 1433-7851            Impact factor:   15.336


  32 in total

Review 1.  Designer proteins: applications of genetic code expansion in cell biology.

Authors:  Lloyd Davis; Jason W Chin
Journal:  Nat Rev Mol Cell Biol       Date:  2012-02-15       Impact factor: 94.444

2.  Genetically encoded tetrazine amino acid directs rapid site-specific in vivo bioorthogonal ligation with trans-cyclooctenes.

Authors:  Jason L Seitchik; Jennifer C Peeler; Michael T Taylor; Melissa L Blackman; Timothy W Rhoads; Richard B Cooley; Christian Refakis; Joseph M Fox; Ryan A Mehl
Journal:  J Am Chem Soc       Date:  2012-02-01       Impact factor: 15.419

Review 3.  Translational recoding in archaea.

Authors:  Beatrice Cobucci-Ponzano; Mosè Rossi; Marco Moracci
Journal:  Extremophiles       Date:  2012-09-27       Impact factor: 2.395

4.  Site-specific protein modifications through pyrroline-carboxy-lysine residues.

Authors:  Weijia Ou; Tetsuo Uno; Hsien-Po Chiu; Jan Grünewald; Susan E Cellitti; Tiffany Crossgrove; Xueshi Hao; Qian Fan; Lisa L Quinn; Paula Patterson; Linda Okach; David H Jones; Scott A Lesley; Ansgar Brock; Bernhard H Geierstanger
Journal:  Proc Natl Acad Sci U S A       Date:  2011-06-13       Impact factor: 11.205

Review 5.  Pyrrolysyl-tRNA synthetase: an ordinary enzyme but an outstanding genetic code expansion tool.

Authors:  Wei Wan; Jeffery M Tharp; Wenshe R Liu
Journal:  Biochim Biophys Acta       Date:  2014-03-12

6.  Genetically encoded norbornene directs site-specific cellular protein labelling via a rapid bioorthogonal reaction.

Authors:  Kathrin Lang; Lloyd Davis; Jessica Torres-Kolbus; Chungjung Chou; Alexander Deiters; Jason W Chin
Journal:  Nat Chem       Date:  2012-02-05       Impact factor: 24.427

7.  A method to site-specifically introduce methyllysine into proteins in E. coli.

Authors:  Hui-Wang Ai; Jae Wook Lee; Peter G Schultz
Journal:  Chem Commun (Camb)       Date:  2010-06-22       Impact factor: 6.222

Review 8.  Distinct genetic code expansion strategies for selenocysteine and pyrrolysine are reflected in different aminoacyl-tRNA formation systems.

Authors:  Jing Yuan; Patrick O'Donoghue; Alex Ambrogelly; Sarath Gundllapalli; R Lynn Sherrer; Sotiria Palioura; Miljan Simonović; Dieter Söll
Journal:  FEBS Lett       Date:  2010-01-21       Impact factor: 4.124

Review 9.  Selenocysteine, pyrrolysine, and the unique energy metabolism of methanogenic archaea.

Authors:  Michael Rother; Joseph A Krzycki
Journal:  Archaea       Date:  2010-08-17       Impact factor: 3.273

10.  Expanding the genetic code of yeast for incorporation of diverse unnatural amino acids via a pyrrolysyl-tRNA synthetase/tRNA pair.

Authors:  Susan M Hancock; Rajendra Uprety; Alexander Deiters; Jason W Chin
Journal:  J Am Chem Soc       Date:  2010-10-27       Impact factor: 15.419
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.