PURPOSE: This case report describes a new clinical method for chair-side processing of a cell mixture which contains mesenchymal stem cells (MSCs) which was applied for the first time in the treatment of a nonunion of an atrophic fractured mandible. METHODS: Bone marrow was aspirated and a corticocancellous bone graft was harvested from the iliac crest of a 56-year-old woman with medical comorbidities and a fracture of the atrophic mandible. The fracture was stabilized with a reconstruction bone plate, and mononuclear cells including MSCs were concentrated by centrifugation and applied in combination with a particulate bone transplant. A sample of the grafted cells was characterized by flow cytometric analysis and by their ability to differentiate into various cell types. RESULTS: The fracture healed uneventfully. No complications occurred during the 4-month follow-up. CONCLUSION: Adding MSCs is a feasible alternative to enhance bone healing. This chair-side method requires little training and no cell laboratory support.
PURPOSE: This case report describes a new clinical method for chair-side processing of a cell mixture which contains mesenchymal stem cells (MSCs) which was applied for the first time in the treatment of a nonunion of an atrophic fractured mandible. METHODS: Bone marrow was aspirated and a corticocancellous bone graft was harvested from the iliac crest of a 56-year-old woman with medical comorbidities and a fracture of the atrophic mandible. The fracture was stabilized with a reconstruction bone plate, and mononuclear cells including MSCs were concentrated by centrifugation and applied in combination with a particulate bone transplant. A sample of the grafted cells was characterized by flow cytometric analysis and by their ability to differentiate into various cell types. RESULTS: The fracture healed uneventfully. No complications occurred during the 4-month follow-up. CONCLUSION: Adding MSCs is a feasible alternative to enhance bone healing. This chair-side method requires little training and no cell laboratory support.
Authors: Elizaveta Kon; Giuseppe Filardo; Alice Roffi; Alessandro Di Martino; Mohammad Hamdan; Laura De Pasqual; Maria Letizia Merli; Maurilio Marcacci Journal: Clin Cases Miner Bone Metab Date: 2012-05-29
Authors: Miguel Padial-Molina; Francisco O'Valle; Alejandro Lanis; Francisco Mesa; David M Dohan Ehrenfest; Hom-Lay Wang; Pablo Galindo-Moreno Journal: Biomed Res Int Date: 2015-05-12 Impact factor: 3.411
Authors: Fabian Duttenhoefer; Stefan F Hieber; Andres Stricker; Rainer Schmelzeisen; Ralf Gutwald; Sebastian Sauerbier Journal: Biores Open Access Date: 2014-04-01