| Literature DB >> 19153666 |
L Yin1, C M Chung, R Huo, H Liu, C Zhou, W Xu, H Zhu, J Zhang, Q Shi, H Y C Wong, J Chen, Y Lu, Y Bi, C Zhao, Y Du, M Ma, Y Cai, W Y Chen, K L Fok, L L Tsang, K Li, Y Ni, Y W Chung, Z Zhou, J Sha, H C Chan.
Abstract
The acrosome reaction has long been thought to be induced by the zona pellucida. Here we report the identification and function of a novel human sperm glycosylphosphatidylinositol (GPI)-anchored membrane protein, NYD-SP8. The release of the protein during sperm-egg interaction and its binding to the cumulus, the first layer of egg investment, elicits cross-talk between the gametes and produces calcium dependant release of progesterone, which lead to the acrosome reaction. An in vivo mouse model of NYD-SP8 immunization is also established showing a reduced fertility rate. Thus, contrary to accepted dogma, our study demonstrates for the first time that, prior to reaching the zona pellucida, sperm may release a surface protein that acts on the cumulus cells leading to the acrosome reaction, which may be important for determining the outcome of fertilization.Entities:
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Year: 2009 PMID： 19153666 DOI： 10.1007/s00018-009-8482-2
Source DB: PubMed Journal: Cell Mol Life Sci ISSN： 1420-682X Impact factor: 9.261