| Literature DB >> 19152866 |
Rebecca Bozym1, Tamiika K Hurst, Nissa Westerberg, Andrea Stoddard, Carol A Fierke, Christopher J Frederickson, Richard B Thompson.
Abstract
This chapter summarizes the use of carbonic anhydrase (CA)-based fluorescent indicators to determine free zinc in solution, in cells, and in subcellular organelles. Expression (both in situ and in vitro) and preparation of CA-based indicators are described, together with techniques of their use, and procedures to minimize contamination. Recipes for zinc buffers are supplied.Mesh:
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Year: 2008 PMID: 19152866 DOI: 10.1016/S0076-6879(08)03414-9
Source DB: PubMed Journal: Methods Enzymol ISSN: 0076-6879 Impact factor: 1.600