Literature DB >> 1915284

The 3 A crystal structure of yeast initiator tRNA: functional implications in initiator/elongator discrimination.

R Basavappa1, P B Sigler.   

Abstract

A significantly improved molecular model of yeast initiator tRNA (ytRNA(iMet) has been prepared that gives insight into the structural basis of eukaryotic initiator tRNA's unique function. This study was made possible by X-ray data collected at synchrotron radiation sources with the newly developed technologies of 'imaging plates' and 'storage phosphors'. These data extend beyond the resolution limit of 4.0 A reported previously to a current limit of 3.0 A and are considerably more accurate. Refinement of the model against the new data (R factor = 21.5%) clearly reveals a novel modification and a set of tertiary interactions involving sequence features characteristic of eukaryotic initiator tRNAs. We hypothesize these to be the structural elements responsible for part of the special function of yeast tRNA(iMET).

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Year:  1991        PMID： 1915284      PMCID： PMC453028          DOI： 10.1002/j.1460-2075.1991.tb07864.x

Source DB:  PubMed          Journal:  EMBO J        ISSN： 0261-4189            Impact factor:   11.598


  22 in total

1.  Presence of phosphorylated O-ribosyl-adenosine in T-psi-stem of yeast methionine initiator tRNA.

Authors:  J Desgrès; G Keith; K C Kuo; C W Gehrke
Journal:  Nucleic Acids Res       Date:  1989-02-11       Impact factor: 16.971

2.  Isolation and characterization of two methionine: tRNA ligases from wheat germ.

Authors:  M D Rosa; P B Sigler
Journal:  Eur J Biochem       Date:  1977-08-15

3.  Crystallographic refinement of yeast aspartic acid transfer RNA.

Authors:  E Westhof; P Dumas; D Moras
Journal:  J Mol Biol       Date:  1985-07-05       Impact factor: 5.469

4.  Three-dimensional structure of Escherichia coli initiator tRNAfMet.

Authors:  N H Woo; B A Roe; A Rich
Journal:  Nature       Date:  1980-07-24       Impact factor: 49.962

5.  The role of modified purine 64 in initiator/elongator discrimination of tRNA(iMet) from yeast and wheat germ.

Authors:  S Kiesewetter; G Ott; M Sprinzl
Journal:  Nucleic Acids Res       Date:  1990-08-25       Impact factor: 16.971

6.  Isoleucyl initiator tRNA does not initiate eucaryotic protein synthesis.

Authors:  T Wagner; M Gross; P B Sigler
Journal:  J Biol Chem       Date:  1984-04-25       Impact factor: 5.157

7.  Yeast tRNAAsp tertiary structure in solution and areas of interaction of the tRNA with aspartyl-tRNA synthetase. A comparative study of the yeast phenylalanine system by phosphate alkylation experiments with ethylnitrosourea.

Authors:  P Romby; D Moras; M Bergdoll; P Dumas; V V Vlassov; E Westhof; J P Ebel; R Giegé
Journal:  J Mol Biol       Date:  1985-08-05       Impact factor: 5.469

8.  Escherichia coli formylmethionine tRNA: mutations in GGGCCC sequence conserved in anticodon stem of initiator tRNAs affect initiation of protein synthesis and conformation of anticodon loop.

Authors:  B L Seong; U L RajBhandary
Journal:  Proc Natl Acad Sci U S A       Date:  1987-01       Impact factor: 11.205

9.  Initiator tRNAs have a unique anticodon loop conformation.

Authors:  P Wrede; N H Woo; A Rich
Journal:  Proc Natl Acad Sci U S A       Date:  1979-07       Impact factor: 11.205

10.  Changing the acceptor identity of a transfer RNA by altering nucleotides in a "variable pocket".

Authors:  W H McClain; K Foss
Journal:  Science       Date:  1988-09-30       Impact factor: 47.728
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  79 in total

1.  The yeast retrotransposon Ty5 uses the anticodon stem-loop of the initiator methionine tRNA as a primer for reverse transcription.

Authors:  N Ke; X Gao; J B Keeney; J D Boeke; D F Voytas
Journal:  RNA       Date:  1999-07       Impact factor: 4.942

2.  An engineered class I transfer RNA with a class II tertiary fold.

Authors:  T A Nissan; B Oliphant; J J Perona
Journal:  RNA       Date:  1999-03       Impact factor: 4.942

3.  Alternative designs for construction of the class II transfer RNA tertiary core.

Authors:  T A Nissan; J J Perona
Journal:  RNA       Date:  2000-11       Impact factor: 4.942

4.  Preparation and activity of synthetic unmodified mammalian tRNAi(Met) in initiation of translation in vitro.

Authors:  T V Pestova; C U Hellen
Journal:  RNA       Date:  2001-10       Impact factor: 4.942

5.  A critical role of water in the specific cleavage of the anticodon loop of some eukaryotic methionine initiator tRNAs.

Authors:  Marcus Perbandt; Miroslawa Z Barciszewska; Christian Betzel; Volker A Erdmann; Jan Barciszewski
Journal:  Mol Biol Rep       Date:  2003-03       Impact factor: 2.316

6.  On the occurrence of the T-loop RNA folding motif in large RNA molecules.

Authors:  Andrey S Krasilnikov; Alfonso Mondragón
Journal:  RNA       Date:  2003-06       Impact factor: 4.942

7.  Identification and modeling of a phosphatase-like domain in a tRNA 2'-O-ribosyl phosphate transferase Rit1p.

Authors:  Anna Czerwoniec; Janusz M Bujnicki
Journal:  Cell Cycle       Date:  2011-10-15       Impact factor: 4.534

8.  Genome-wide analysis of N1-methyl-adenosine modification in human tRNAs.

Authors:  Mridusmita Saikia; Ye Fu; Mariana Pavon-Eternod; Chuan He; Tao Pan
Journal:  RNA       Date:  2010-05-19       Impact factor: 4.942

9.  Nuclear RNA surveillance in Saccharomyces cerevisiae: Trf4p-dependent polyadenylation of nascent hypomethylated tRNA and an aberrant form of 5S rRNA.

Authors:  Sujatha Kadaba; Xuying Wang; James T Anderson
Journal:  RNA       Date:  2006-01-23       Impact factor: 4.942

10.  Trmt61B is a methyltransferase responsible for 1-methyladenosine at position 58 of human mitochondrial tRNAs.

Authors:  Takeshi Chujo; Tsutomu Suzuki
Journal:  RNA       Date:  2012-10-24       Impact factor: 4.942
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