Aila Leino1, M K Koivula. 1. TYKSLAB, the Hospital District of Southwest Finland, Turku, Finland. aila.leino@tyks.fi
Abstract
BACKGROUND: The stability of analyte concentrations in plasma after prolonged contact with blood cells in uncentrifuged lithium-heparin gel tubes was studied. METHODS: To investigate the stability of concentrations of 26 chemistry and 15 immunochemistry analytes, the simultaneously drawn samples (n = 50) were measured after 6 h storage at +8 degrees C and +22 degrees C in whole blood and after immediate separation of plasma. The analyte concentrations were measured with a Roche Modular PPEE analyser using reagents from Roche Diagnostics. RESULTS: After prolonged contact with cells a clinically significant change was only observed for potassium where the mean value increased from 4.0 mmol/L to 4.8 mmol/L (P < 0.001) when stored at +8 degrees C. CONCLUSION: Immediate separation of plasma from cells is recommended. However, when prolonged contact of plasma with cell is unavoidable, samples can be kept uncentrifuged for up to 6 h at +8 degrees C or at +22 degrees C. The stability of potassium, however, is temperature-dependent and cannot be measured from refrigerated blood samples.
BACKGROUND: The stability of analyte concentrations in plasma after prolonged contact with blood cells in uncentrifuged lithium-heparin gel tubes was studied. METHODS: To investigate the stability of concentrations of 26 chemistry and 15 immunochemistry analytes, the simultaneously drawn samples (n = 50) were measured after 6 h storage at +8 degrees C and +22 degrees C in whole blood and after immediate separation of plasma. The analyte concentrations were measured with a Roche Modular PPEE analyser using reagents from Roche Diagnostics. RESULTS: After prolonged contact with cells a clinically significant change was only observed for potassium where the mean value increased from 4.0 mmol/L to 4.8 mmol/L (P < 0.001) when stored at +8 degrees C. CONCLUSION: Immediate separation of plasma from cells is recommended. However, when prolonged contact of plasma with cell is unavoidable, samples can be kept uncentrifuged for up to 6 h at +8 degrees C or at +22 degrees C. The stability of potassium, however, is temperature-dependent and cannot be measured from refrigerated blood samples.
Authors: Gabriel Lima-Oliveira; Giuseppe Lippi; Gian Luca Salvagno; Matteo Gelati; Antonella Bassi; Alberto Contro; Francesca Pizzolo; Gian Cesare Guidi Journal: Biochem Med (Zagreb) Date: 2016-10-15 Impact factor: 2.313
Authors: Carlos Fernando Yauli Flores; Ángela de Las Mercedes Hurtado Pineda; Victoria Maritza Cevallos Bonilla; Klever Sáenz-Flor Journal: EJIFCC Date: 2020-03-20