| Literature DB >> 19141594 |
Jung-Hyun Kim1, Thomas Ebersole, Natalay Kouprina, Vladimir N Noskov, Jun-Ichirou Ohzeki, Hiroshi Masumoto, Brankica Mravinac, Beth A Sullivan, Adam Pavlicek, Sinisa Dovat, Svetlana D Pack, Yoo-Wook Kwon, Patrick T Flanagan, Dmitri Loukinov, Victor Lobanenkov, Vladimir Larionov.
Abstract
The role of repetitive DNA sequences in pericentromeric regions with respect to kinetochore/heterochromatin structure and function is poorly understood. Here, we use a mouse erythroleukemia cell (MEL) system for studying how repetitive DNA assumes or is assembled into different chromatin structures. We show that human gamma-satellite DNA arrays allow a transcriptionally permissive chromatin conformation in an adjacent transgene and efficiently protect it from epigenetic silencing. These arrays contain CTCF and Ikaros binding sites. In MEL cells, this gamma-satellite DNA activity depends on binding of Ikaros proteins involved in differentiation along the hematopoietic pathway. Given our discovery of gamma-satellite DNA in pericentromeric regions of most human chromosomes and a dynamic chromatin state of gamma-satellite arrays in their natural location, we suggest that gamma-satellite DNA represents a unique region of the functional centromere with a possible role in preventing heterochromatin spreading beyond the pericentromeric region.Entities:
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Year: 2009 PMID： 19141594 PMCID： PMC2665773 DOI： 10.1101/gr.086496.108
Source DB: PubMed Journal: Genome Res ISSN： 1088-9051 Impact factor: 9.043