AIMS: The Archaea diversity was evaluated in an agricultural biogas plant supplied with cattle liquid manure and maize silage under mesophilic conditions. METHODS AND RESULTS: Two different genes (16S rRNA; methyl-coenzyme-M-reductase, MCR) targeted by three different PCR primer sets were selected and used for the construction of three clone libraries comprising between 104 and 118 clones. The clone libraries were analysed by restriction fragment polymorphism (RFLP). Between 11 and 31 operational taxonomic units (OTUs) were detected and assigned to orders Methanomicrobiales, Methanosarcinales and Methanobacteriales. Over 70% of all Archaea OTUs belong to the order Methanomicrobiales which mostly include hydrogenotrophic methanogens. Acetotrophic methanogens were detected in minor rates. Similar relative values were obtained by a quantitative real-time PCR analysis. CONCLUSIONS: The results implied that in this biogas plant the most of the methane formation resulted from the conversion of H(2) and CO(2). SIGNIFICANCE AND IMPACT OF THE STUDY: This study reports, for the first time, a molecular analysis of the archaeal community in this type of agricultural biogas plants. Therein the hydrogenotrophic methanogenesis seems to be the major pathway of methane formation. These results are in contrast with the common thesis that in biogas fermentations the primary substrate for methanogenesis is acetate.
AIMS: The Archaea diversity was evaluated in an agricultural biogas plant supplied with cattle liquid manure and maize silage under mesophilic conditions. METHODS AND RESULTS: Two different genes (16S rRNA; methyl-coenzyme-M-reductase, MCR) targeted by three different PCR primer sets were selected and used for the construction of three clone libraries comprising between 104 and 118 clones. The clone libraries were analysed by restriction fragment polymorphism (RFLP). Between 11 and 31 operational taxonomic units (OTUs) were detected and assigned to orders Methanomicrobiales, Methanosarcinales and Methanobacteriales. Over 70% of all Archaea OTUs belong to the order Methanomicrobiales which mostly include hydrogenotrophic methanogens. Acetotrophic methanogens were detected in minor rates. Similar relative values were obtained by a quantitative real-time PCR analysis. CONCLUSIONS: The results implied that in this biogas plant the most of the methane formation resulted from the conversion of H(2) and CO(2). SIGNIFICANCE AND IMPACT OF THE STUDY: This study reports, for the first time, a molecular analysis of the archaeal community in this type of agricultural biogas plants. Therein the hydrogenotrophic methanogenesis seems to be the major pathway of methane formation. These results are in contrast with the common thesis that in biogas fermentations the primary substrate for methanogenesis is acetate.
Authors: E Nettmann; I Bergmann; S Pramschüfer; K Mundt; V Plogsties; C Herrmann; M Klocke Journal: Appl Environ Microbiol Date: 2010-02-12 Impact factor: 4.792
Authors: David Wilkins; Xiao-Ying Lu; Zhiyong Shen; Jiapeng Chen; Patrick K H Lee Journal: Appl Environ Microbiol Date: 2014-11-07 Impact factor: 4.792
Authors: Monika Vítězová; Jan Lochman; Martina Zapletalová; Stefan Ratering; Sylvia Schnell; Tomáš Vítěz Journal: World J Microbiol Biotechnol Date: 2021-10-06 Impact factor: 3.312
Authors: Christian Abendroth; Cristina Vilanova; Thomas Günther; Olaf Luschnig; Manuel Porcar Journal: Biotechnol Biofuels Date: 2015-06-18 Impact factor: 6.040
Authors: K L Kovács; N Ács; E Kovács; R Wirth; G Rákhely; Orsolya Strang; Zsófia Herbel; Z Bagi Journal: Biomed Res Int Date: 2012-12-31 Impact factor: 3.411