Z Rao1, Z Ma, W Shen, H Fang, J Zhuge, X Wang. 1. The Key Laboratory of Industrial Biotechnology of Ministry of Education, Research Center of Industrial Microbiology, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu Province, PR China. raozm@yahoo.com.cn
Abstract
AIMS: Saccharomyces cerevisiae is a safe micro-organism used in fermentation industry. 1,3-Propanediol is an important chemical widely used in polymer production, but its availability is being restricted owing to its expensively chemical synthesis. The aim of this study is to engineer a S. cerevisiae strain that can produce 1,3-propanediol at low cost. METHODS AND RESULTS: By using D-glucose as a feedstock, S. cerevisiae could produce glycerol, but not 1,3-propanediol. In this study, we have cloned two genes yqhD and dhaB required for the production of 1,3-propanediol from glycerol, and integrated them into the chromosome of S. cerevisiae W303-1A by Agrobacterium tumefaciens-mediated transformation. Both genes yqhD and dhaB functioned in the engineered S. cerevisiae and led to the production of 1,3-propanediol from D-glucose. CONCLUSION: Saccharomyces cerevisiae can be engineered to produce 1,3-propanediol from low-cost feedstock D-glucose. SIGNIFICANCE AND IMPACT OF THE STUDY: To our knowledge, this is the first report on developing S. cerevisiae to produce 1,3-propanediol by using A. tumefaciens-mediated transformation. This study might lead to a safe and cost-efficient method for industrial production of 1,3-propanediol.
AIMS: Saccharomyces cerevisiae is a safe micro-organism used in fermentation industry. 1,3-Propanediol is an important chemical widely used in polymer production, but its availability is being restricted owing to its expensively chemical synthesis. The aim of this study is to engineer a S. cerevisiae strain that can produce 1,3-propanediol at low cost. METHODS AND RESULTS: By using D-glucose as a feedstock, S. cerevisiae could produce glycerol, but not 1,3-propanediol. In this study, we have cloned two genes yqhD and dhaB required for the production of 1,3-propanediol from glycerol, and integrated them into the chromosome of S. cerevisiae W303-1A by Agrobacterium tumefaciens-mediated transformation. Both genes yqhD and dhaB functioned in the engineered S. cerevisiae and led to the production of 1,3-propanediol from D-glucose. CONCLUSION:Saccharomyces cerevisiae can be engineered to produce 1,3-propanediol from low-cost feedstock D-glucose. SIGNIFICANCE AND IMPACT OF THE STUDY: To our knowledge, this is the first report on developing S. cerevisiae to produce 1,3-propanediol by using A. tumefaciens-mediated transformation. This study might lead to a safe and cost-efficient method for industrial production of 1,3-propanediol.