OBJECTIVES: To compare different conditioning regimens (CR), in order to determine whether either of them could be less toxic to allogeneic bone marrow transplantation (BMT) patients in terms of oxidative stress and also analyze delta-ALA-D activity as a possible marker of oxidative stress. DESIGN AND METHODS: Lipid peroxidation, vitamin C, thiol groups levels and catalase, superoxide dismutase and delta-ALA-D activity were determined in 21 healthy controls, 5 patients with fludarabine+cyclophosphamide (FluCy) CR, 12 with busulfan+cyclophosphamide (BuCy) and 4 with cyclophosphamide+total body irradiation (CyTBI). RESULTS: There were a decrease in enzymatic and non enzymatic antioxidants, in delta-ALA-D activity, and in all CRs and an increase in lipid peroxidation more pronounced in CyTBI CR. CONCLUSIONS: All CRs promoted oxidative stress in allogeneic BMT patients, but this was more pronounced with CyTBI and delta-ALA-D activity seemed to be an additional biomarker of oxidative stress in these patients.
OBJECTIVES: To compare different conditioning regimens (CR), in order to determine whether either of them could be less toxic to allogeneic bone marrow transplantation (BMT) patients in terms of oxidative stress and also analyze delta-ALA-D activity as a possible marker of oxidative stress. DESIGN AND METHODS: Lipid peroxidation, vitamin C, thiol groups levels and catalase, superoxide dismutase and delta-ALA-D activity were determined in 21 healthy controls, 5 patients with fludarabine+cyclophosphamide (FluCy) CR, 12 with busulfan+cyclophosphamide (BuCy) and 4 with cyclophosphamide+total body irradiation (CyTBI). RESULTS: There were a decrease in enzymatic and non enzymatic antioxidants, in delta-ALA-D activity, and in all CRs and an increase in lipid peroxidation more pronounced in CyTBI CR. CONCLUSIONS: All CRs promoted oxidative stress in allogeneic BMT patients, but this was more pronounced with CyTBI and delta-ALA-D activity seemed to be an additional biomarker of oxidative stress in these patients.
Authors: Thissiane L Gonçalves; Dalila M Benvegnú; Gabriela Bonfanti; Andressa V Frediani; João Batista T Rocha Journal: BMC Cancer Date: 2009-05-08 Impact factor: 4.430
Authors: Osmel La-Llave-León; Edna M Méndez-Hernández; Francisco X Castellanos-Juárez; Eloísa Esquivel-Rodríguez; Fernando Vázquez-Alaniz; Ada Sandoval-Carrillo; Gonzalo García-Vargas; Jaime Duarte-Sustaita; Jorge L Candelas-Rangel; José M Salas-Pacheco Journal: Int J Environ Res Public Health Date: 2017-04-18 Impact factor: 3.390