Literature DB >> 19109478

Detection and toxin typing of Clostridium perfringens in formalin-fixed, paraffin-embedded tissue samples by PCR.

Josephine Wu1, Wandi Zhang, Boxun Xie, Maoxin Wu, Xiaodi Tong, Jayant Kalpoe, David Zhang.   

Abstract

Since current microbiology methods are not suitable to detect Clostridium perfringens in formalin-fixed, paraffin-embedded tissue samples, we developed a PCR assay to detect toxin-encoding genes and the 16S rRNA gene of C. perfringens. We successfully detected and genotyped C. perfringens in tissue sections from two autopsy cases.

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Year:  2008        PMID: 19109478      PMCID: PMC2650962          DOI: 10.1128/JCM.01324-08

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN: 0095-1137            Impact factor:   5.948


  15 in total

1.  Detection of the etx gene (epsilon-toxin inducer) in plasmids of high molecular weight in Clostridium perfringens type D.

Authors:  A B Bentancor; M R Fermepín; L D Bentancor; R A de Torres
Journal:  FEMS Immunol Med Microbiol       Date:  1999-07

Review 2.  Toxigenic clostridia.

Authors:  C L Hatheway
Journal:  Clin Microbiol Rev       Date:  1990-01       Impact factor: 26.132

3.  Detection of hepatitis C virus RNA using ligation-dependent polymerase chain reaction in formalin-fixed, paraffin-embedded liver tissues.

Authors:  Y N Park; K Abe; H Li; T Hsuih; S N Thung; D Y Zhang
Journal:  Am J Pathol       Date:  1996-11       Impact factor: 4.307

4.  Molecular typing and epidemiological survey of prevalence of Clostridium perfringens types by multiplex PCR.

Authors:  H S Yoo; S U Lee; K Y Park; Y H Park
Journal:  J Clin Microbiol       Date:  1997-01       Impact factor: 5.948

5.  Enteritis necroticans (pigbel) in a diabetic child.

Authors:  T M Petrillo; C M Beck-Sagué; J G Songer; C Abramowsky; J D Fortenberry; L Meacham; A G Dean; H Lee; D M Bueschel; S R Nesheim
Journal:  N Engl J Med       Date:  2000-04-27       Impact factor: 91.245

6.  Phylogenetic analysis of phospholipase C genes from Clostridium perfringens types A to E and Clostridium novyi.

Authors:  K Tsutsui; J Minami; O Matsushita; S Katayama; Y Taniguchi; S Nakamura; M Nishioka; A Okabe
Journal:  J Bacteriol       Date:  1995-12       Impact factor: 3.490

7.  Comparison of DNA and RNA extraction methods for mummified tissues.

Authors:  Nami Konomi; Eve Lebwohl; David Zhang
Journal:  Mol Cell Probes       Date:  2002-12       Impact factor: 2.365

8.  A 16S rDNA-based PCR method for rapid and specific detection of Clostridium perfringens in food.

Authors:  R F Wang; W W Cao; W Franklin; W Campbell; C E Cerniglia
Journal:  Mol Cell Probes       Date:  1994-04       Impact factor: 2.365

9.  Comparison of the nucleotide sequence and development of a PCR test for the epsilon toxin gene of Clostridium perfringens type B and type D.

Authors:  H L Havard; S E Hunter; R W Titball
Journal:  FEMS Microbiol Lett       Date:  1992-10-01       Impact factor: 2.742

10.  Virulence studies on chromosomal alpha-toxin and theta-toxin mutants constructed by allelic exchange provide genetic evidence for the essential role of alpha-toxin in Clostridium perfringens-mediated gas gangrene.

Authors:  M M Awad; A E Bryant; D L Stevens; J I Rood
Journal:  Mol Microbiol       Date:  1995-01       Impact factor: 3.501

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  15 in total

1.  Macrophage migration inhibitory factor enzymatic activity, lung inflammation, and cystic fibrosis.

Authors:  Huzaifa Adamali; Michelle E Armstrong; Anne Marie McLaughlin; Gordon Cooke; Edward McKone; Christine M Costello; Charles G Gallagher; Lin Leng; John A Baugh; Günter Fingerle-Rowson; Richard J Bucala; Paul McLoughlin; Seamas C Donnelly
Journal:  Am J Respir Crit Care Med       Date:  2012-05-16       Impact factor: 21.405

2.  Epidemiological and pathobiological profiles of Clostridium perfringens infections: review of consecutive series of 33 cases over a 13-year period.

Authors:  Yuji Shindo; Yoh Dobashi; Toshiyasu Sakai; Chie Monma; Hiroyuki Miyatani; Yukio Yoshida
Journal:  Int J Clin Exp Pathol       Date:  2015-01-01

3.  Genomic diversity of Clostridium perfringens strains isolated from food and human sources.

Authors:  A Afshari; A Jamshidi; J Razmyar; M Rad
Journal:  Iran J Vet Res       Date:  2016       Impact factor: 1.376

4.  Diversity of toxin genotypes and antimicrobial susceptibility of Clostridium perfringens isolates from feces of infants.

Authors:  Alaa J Al Radaideh; Eman F Badran; Asem A Shehabi
Journal:  Germs       Date:  2019-03-01

5.  Clostridium difficile isolates with increased sporulation: emergence of PCR ribotype 002 in Hong Kong.

Authors:  V C C Cheng; W C Yam; O T C Lam; J L Y Tsang; E Y F Tse; G K H Siu; J F W Chan; H Tse; K K W To; J W M Tai; P L Ho; K Y Yuen
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2011-04-06       Impact factor: 3.267

6.  Sensitive quantification of Clostridium perfringens in human feces by quantitative real-time PCR targeting alpha-toxin and enterotoxin genes.

Authors:  Ravinder Nagpal; Kiyohito Ogata; Hirokazu Tsuji; Kazunori Matsuda; Takuya Takahashi; Koji Nomoto; Yoshio Suzuki; Kazunari Kawashima; Satoru Nagata; Yuichiro Yamashiro
Journal:  BMC Microbiol       Date:  2015-10-19       Impact factor: 3.605

7.  Experimental induction of necrotic enteritis in chickens by a netB-positive Japanese isolate of Clostridium perfringens.

Authors:  Ho To; Takayuki Suzuki; Fumiya Kawahara; Koji Uetsuka; Shinya Nagai; Tetsuo Nunoya
Journal:  J Vet Med Sci       Date:  2016-12-15       Impact factor: 1.267

8.  Clostridium Perfringens Infection in a Febrile Patient with Severe Hemolytic Anemia.

Authors:  Masamitsu Hashiba; Atsutoshi Tomino; Nobuyoshi Takenaka; Tomonori Hattori; Hideki Kano; Masanobu Tsuda; Naoshi Takeyama
Journal:  Am J Case Rep       Date:  2016-04-06

9.  A case of nontraumatic gas gangrene in a common marmoset (Callithrix jacchus).

Authors:  Masahiko Yasuda; Takashi Inoue; Masami Ueno; Hanako Morita; Nobuhito Hayashimoto; Kenji Kawai; Toshio Itoh
Journal:  J Vet Med Sci       Date:  2015-07-09       Impact factor: 1.267

10.  Genotyping of Clostridium perfringens isolated from broiler meat in northeastern of Iran.

Authors:  Asma Afshari; Abdollah Jamshidi; Jamshid Razmyar; Mehrnaz Rad
Journal:  Vet Res Forum       Date:  2015-12-15       Impact factor: 1.054

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